Odorant receptor orthologues from moths display conserved responses to cis-jasmone  

作　　者：Xiao-Qing Hou Zhongqiang Jia Dan-Dan Zhang Guirong Wang 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong Province,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China [3]Department of Biology,Lund University,Lund,Sweden

出　　处：《Insect Science》2024年第4期1107-1120,共14页昆虫科学（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.32202307,32130089);Shenzhen Science and Technology Program(Grant No.KQTD20180411143628272);Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District(Grant No.PT202101-02);the China Postdoctoral Science Foundation(Grant No.2021M703548).

摘　　要：In insects,the odorant receptor(OR)multigene family evolves by the birth-and-death evolutionary model,according to which the OR repertoire of each species has undergone specific gene gains and losses depending on their chemical environment,resulting in taxon-specific OR lineage radiations with different sizes in the phylogenetic trees.Despite the general divergence in the gene family across different insect orders,the ORs in moths seem to be genetically conserved across species,clustered into 23 major clades containing multiple orthologous groups with single-copy gene from each species.We hypothesized that ORs in these orthologous groups are tuned to ecologically important compounds and functionally conserved.cis-Jasmone is one of the compounds that not only primes the plant defense of neighboring receiver plants,but also functions as a behavior regulator to various insects.To test our hypothesis,using Xenopus oocyte recordings,we functionally assayed the orthologues of BmorOR56,which has been characterized as a specific receptor for cis-jasmone.Our results showed highly conserved response specificity of the BmorOR56 orthologues,with all receptors within this group exclusively responding to cis-jasmone.This is supported by the dN/dS analysis,showing that strong purifying selection is acting on this group.Moreover,molecular docking showed that the ligand binding pockets of BmorOR56 orthologues to cis-jasmone are similar.Taken together,our results suggest the high conservation of OR for ecologically important compounds across Heterocera.

关 键 词：CIS-JASMONE evolutionary conservation functional characterization molec-ular docking MOTHS purifying selection 

分 类 号：Q96[生物学—昆虫学]