Egg-associated secretions from the brown planthopper(Nilaparvata lugens)activate rice immune responses  

作　　者：Jing Li Shuai Li Jing Li Xinyang Tan Zhichang Zhao Lei Jiang Ary A.Hoffmann Jichao Fang Rui Ji 

机构地区：[1]Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Food and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Nanjing,China [2]College of Plant Protection,Nanjing Agricultural University,Nanjing,China [3]School of Plant Protection,Anhui Agricultural University,Hefei,China [4]School of BioSciences,Bio21 Institute,University of Melbourne,Parkville,VIC,Australia [5]Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection,Huaiyin Normal University,Huaian,Jiangsu Province,China

出　　处：《Insect Science》2024年第4期1135-1149,共15页昆虫科学（英文版）

基　　金：supported by the Jiangsu Agricultural Science and Technology Independent Innovation Fund[CX(22)3018];the National Natural Science Foundation of China(32302320);the National Key Basic Research Program of China(2021YFD1401100).

摘　　要：The brown planthopper(BPH,Nilaparvata lugens)is a notorious sap-sucking insect pest that damages rice(Oryza sativa)plants throughout Asia.During BPH feeding,saliva enters rice plant tissues,whereas during oviposition egg-associated secretions(EAS)are deposited in damaged plant tissue.Dynamic changes in rice to planthopper salivary effectors have been widely reported.However,the effects of EAS from planthopper on rice immunity remains largely unexplored.In this study,we found that both infestation of rice by gravid BPH female adults and treatment with the EAS elicited a strong and rapid accumulation of jasmonic acid(JA),JA-isoleucine,and hydrogen peroxide in rice.EAS enhanced plant defenses not only in rice but also in tobacco,and these impaired the performance of BPH on rice,as well as the performance of aphids and whiteflies on tobacco.High-throughput proteome sequencing of EAS led to 110 proteins being identified and 53 proteins with 2 or more unique peptides being detected.Some proteins from BPH EAS were also found in the salivary proteome from herbivores,suggesting potential evolutionary conservation of effector functions across feeding and oviposition;however,others were only identified in EAS,and these are likely specifically related to oviposition.These findings point to novel proteins affecting interactions between planthoppers and rice during oviposition,providing an additional source of information for effector studies.

关 键 词：egg-associated secretion Nilaparvata lugens OVIPOSITION plant immunity proteome 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]