Highly efficient expression of human extracellular superoxide dismutase(rhEcSOD)with ultraviolet-B-induced damage-resistance activity in transgenic silkworm cocoons  

作　　者：Feng Wang Ri-Yuan Wang De-Bin Zhong Ping Zhao Qing-You Xia 

机构地区：[1]Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City,Biological Science Research Center,Southwest University,Chongqing,China [2]Century Legend Biotechnology Research Institute(Chongqing)Co.,Ltd.,Chongqing,China

出　　处：《Insect Science》2024年第4期1150-1164,共15页昆虫科学（英文版）

基　　金：supported by the National Key Research and Development Program of China(2022YFD1201600);Chongqing Science and Technology Commission(cstc2020jcyj-cxttX0001,CSTB2022TIAD-KPX0083);Yongchuan District Science and Technology Commission(2022ycyfjg20002).

摘　　要：Extracellular superoxide dismutase(EcSOD)protects tissues from oxidative stress,and thus is considered as a therapeutic agent for many diseases such as atherosclerosis,hypertension,and cancer.However,cost-effective production of bioactive recombinant human EcSOD(rhEcSOD)remains a challenge.Herein,we developed an efficient strategy for producing active rhEcSOD by transgenic silkworms.rhEcSOD was successfully synthesized as homodimers and homotetramers in the middle silk gland and spun into the cocoons with a concentration of 9.48±0.21 mg/g.Purification of rhEcSOD from the cocoons could be conveniently achieved with a purity of 99.50%and a yield of 3.5±0.5 mg/g.Additionally,N-glycosylation at the only site of N89 in rhEcSOD with 10 types were identified.The purified rhEcSOD gained the potent enzymatic activity of 4162±293 U/mg after Cu/Zn ions incorporation.More importantly,rhEcSOD was capable of penetrating and accumulating in the nuclei of cells to maintain cell morphology and attenuate ultraviolet B-induced cell apoptosis by eliminating reactive oxygen species and inhibiting the C-Jun N-terminal kinase signaling pathway.These results demonstrated that the transgenic silkworm could successfully produce rhEcSOD with enzymatic and biological activities for biomedical applications.

关 键 词：ANTIOXIDANT human extracellular superoxide dismutase recombinant expression silkworm silk gland 

分 类 号：Q96[生物学—昆虫学]