n-octyl acrylate is a candidate sex pheromone component involved in courtship in parasitoid wasp Microplitis mediator  

作　　者：Yu-Xiao Tao Shuang Shan Youssef Dewer Shan-Ning Wang Adel Khashaveh Rui-Jun Li Yong-Jun Zhang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China [2]College of Plant Protection,Agricultural University of Hebei,Baoding,Hebei Province,China [3]Bioassay Research Department,Central Agricultural Pesticide Laboratory,Sabahia Plant Protection Research Station,Agricultural Research Center,Alexandria,Egypt [4]Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China,Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing,China

出　　处：《Insect Science》2024年第4期1200-1210,共11页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(32150410366,31972338,and 32372639);China Agriculture Research System(CARS-02-26);the National Key Research and Development Program of China(2021YFD1400700).

摘　　要：Sex pheromones are considered to play critical roles in partner communication of most parasitic Hymenoptera.However,the identification of sex pheromone components remains limited to a few families of parasitoid wasps.In this study,we functionally characterized a candidate sex pheromone component in Microplitis mediator(Hymenoptera:Braconidae),a solitary parasitoid of Noctuidae insects.We found that the body surface extract from female wasps could significantly stimulate courtship behavior of males.Gas chromatography-electroantennographic detection(GC-EAD)analysis revealed that a candidate semiochemical from extract triggered significant electrophysiological response of antennae of males.By performing gas chromatography-mass spectrometer(GC-MS)measurement,GC-EAD active compound was identified as n-octyl acrylate,a candidate sex pheromone component in female M.mediator.In electroantennogram(EAG)tests,antennae of male wasps showed significantly higher electrophysiological responses to n-octyl acrylate than those of females.Y-tube olfactometer assays indicated that male wasps significantly chose n-octyl acrylate compared with the control.Furthermore,male wasps showed a remarkable preference for n-octyl acrylate in a simulated field condition behavioral trial;simultaneously,n-octyl acrylate standard could also trigger significant courtship behavior in males.We propose that n-octyl acrylate,as a candidate vital sex pheromone component,could be utilized to design behavioral regulators of M.mediator to implement the protection and utilization of natural enemies.

关 键 词：behavioral assay courtship behavior GC-EAD analysis Microplitis mediator sex pheromone 

分 类 号：Q96[生物学—昆虫学]