Fatty acids derived from oviposition systems guide female black soldier flies(Hermetia illucens)toward egg deposition sites  

作　　者：Patrick Klüber Emna Arous Jeanny Jerschow Marco Fraatz Daniel Bakonyi Martin Rühl Holger Zorn 

机构地区：[1]Department Bioresources,Fraunhofer Institute for Molecular Biology and Applied Ecology,Giessen,Germany [2]Institute of Food Chemistry and Food Biotechnology,Justus Liebig University Giessen,Giessen,Germany

出　　处：《Insect Science》2024年第4期1231-1248,共18页昆虫科学（英文版）

基　　金：supported by grants from the Hessian Ministry of Higher Education,Research and the Arts via the LOEWE Center for Insect Biotechnology;by the German Federal Ministry for Education and Research(BMBF)grant FKZ 031B1291B(InA)within the BioBall innovation space;Open access funding enabled and organized by Projekt DEAL.

摘　　要：The black soldier fly,Hermetia illucens,comes with big promises for industrial purposes since its larvae feed polyphagously on a broad spectrum of organic substrates.However,research focusing on adult flies is scarce,which is inconsistent with their reproductive relevance within the rearing cycle.In particular,directed oviposition is a challenge in artificial systems.Currently,decomposing organic matter is commonly used as oviposition substrate,which has extensive potential for improvement in view of the lack of standardization and the risk of microbial contamination.Here,we identified three fatty acids and one fatty acid methyl ester derived from the surface of old oviposition sites and targeted to elucidate their effect on preference behavior and oviposition site selection using Y-olfactometry and prepared oviposition sites,respectively.Exposure to tetradecanoic acid attracted gravid females and stimulated oviposition most strongly,while decanoic acid demonstrated a repulsive effect.Females kept in mixed-sex populations were attracted by tetradecanoic acid,resulting in a higher egg mass found in the compound box(3.0–11.4 fold),a≥2.3 fold reduction of nonspecifically deposited eggs,and the highest total egg mass.Conversely,decanoic and dodecanoic acid caused females to lay a greater proportion of eggs nonspecifically outside both boxes.Our data suggest that fatty acids,especially tetradecanoic acid,are important cues for oviposition site selection in black soldier flies.In order to achieve a directed oviposition behavior,the role of further short-and long-chain fatty acids as attractants should be examined.

关 键 词：black soldier fly Hermetia illucens fatty acids insects as food and feed OVIPOSITION oviposition site selection tetradecanoic acid 

分 类 号：Q96[生物学—昆虫学]