12株桑源克雷伯菌属细菌耐药性、毒力基因及分子分型分析  

Analysis of drug resistance,virulence genes and molecular typing of 12 strains of Klebsiella spp.of mulberry

作　　者：张雪吟 罗龙辉 刘吉平[1] Zhang Xueyin;Luo Longhui;Liu Jiping(College of Animal Science,South China Agricultural University,Guangdong Provincial Key Laboratory of Agricultural Animal Genomics and Molecular Breeding,Guangzhou 510642;College of Food Science,South China Agricultural University,Guangzhou 510642;Population Microbiology Center,South China Agricultural University,Guangzhou 510642)

机构地区：[1]华南农业大学动物科学学院,广东省农业动物基因组学与分子育种重点实验室,广州510642 [2]华南农业大学食品学院,广州510642 [3]华南农业大学群体微生物中心,广州510642

出　　处：《中国抗生素杂志》2024年第7期823-831,共9页Chinese Journal of Antibiotics

基　　金：财政部和农业农村部:国家现代农业产业技术体系(蚕桑)。

摘　　要：目的为了解不同来源的人类条件病原体的特征和耐药机制,预防环境病原体和医院病原体交叉感染,对桑树中分离的克雷伯菌进行耐药性、毒力基因、分子分型特点的探究。方法从广东和广西两地采集桑树和桑树根际土壤样本,其中共分离得到12株克雷伯菌,对其采用纸片扩散法(K-B法)检测菌株耐药性表征;采用PCR技术对6类9个抗生素相关耐药基因和毒力基因进行检测;采用多位点序列分型(multilocus sequence typing,MLST)技术进行遗传分析。结果12株桑源克雷伯菌检测到bla_(SHV)、tet(D)、tet(A)和aadA14种耐药基因,其中携带氨基糖苷类相关基因aadA1的有5株(41.67%);12株桑源克雷伯菌均对氨基糖苷类抗生素红霉素和链霉素表现出耐药,对6种以上抗生素耐药的菌株有6株(50%),对7种抗生素耐药的菌株有2株(16.67%);8株(66.67%)携带脂多糖相关的wabG毒力基因;MLST分析显示12株桑源克雷伯菌划分为3大类,包括产酸克雷伯菌复合体(Klebsiella oxytoca species complex,KoSC)、肺炎克雷伯菌复合体(K.pneumoniae species complex,KpSC)以及产气克雷伯菌(K.aerogenes);可分成7个序列型(sequence typing,ST),ST260为优势序列型,有5株(41.67%)。结论12株桑源克雷伯菌对氨基糖苷类抗生素耐药情况严峻,氨基糖苷类aadA1耐药基因检出率最高;ST260是主要的序列型,菌株之间存在遗传多样性,为环境中桑树来源的克雷伯菌病原体致病机制提供理论依据。Objective To understand the characteristics and resistance mechanisms of human conditioned pathogens from different sources,and to prevent cross-infection between environmental pathogens and nosocomial pathogens,the characteristics of drug resistance,virulence genes and molecular typing of Klebsiella isolated from mulberry trees were explored.Methods A total of 12 strains of Klebsiella were isolated from mulberry and mulberry rhizosphere soil samples from Guangdong and Guangxi,and the strain resistance was characterized by the K-B method.PCR was used for the detection of 6 classes of nine antibiotic-associated resistance genes and virulence genes,and genetic analysis was performed by multilocus sequence typing(MLST).Results Four resistance genes,bla_(SHV),tet(D),tet(A)and aadA1,were detected in 12 strains of Klebsiella mulberryensis,of which 5 strains(41.67%)carried the aminoglycoside-related gene aadA1.The 12 strains of Klebsiella from mulberry were resistant to aminoglycoside antibiotics,erythromycin and streptomycin,and 6 strains(50%)were resistant to 6 or more antibiotics and 6 strains(50%)were resistant to 7 or more antibiotics,and 2 strains(16.67%)resistant to 7 antibiotics;8 strains(66.67%)carried lipopolysaccharide-associated wabG virulence genes;MLST analysis showed that the 12 strains of mulberry-derived Klebsiella were classified into 3 major groups,including acid-producing Klebsiella oxytoca species complex(KoSC),K.pneumoniae species complex(KpSC),and K.aerogenes;they could be classified into seven sequence typing(ST),and ST260 was the dominant sequence typing,with five strains(41.67%).Conclusion The 12 strains of Klebsiella spp.of mulberry were severely resistant to aminoglycoside antibiotics,and the aminoglycoside aadA1 resistance gene was detected at the highest rate;ST260 was the predominant sequence type,and there was genetic diversity among the strains,which provided theoretical basis for the mechanism of pathogenicity of Klebsiella spp.pathogens originating from mulberry trees in the environmen

关 键 词：克雷伯菌属 条件致病菌 耐药性 毒力基因 ST分型 

分 类 号：R378.996[医药卫生—病原生物学]