茉莉酸合成基因正向调控番茄对晚疫病的阶段性抗性  

Jasmonic acid biosynthetic genes positively regulate age-related resistance of tomato plants to Phytophthora infestans

作　　者：江玉霞 代丽丽[2,5] 薛晓婧 于悦 张建荔 丁新华 钟巍然[4] 储昭辉[3] 刘海峰 JIANG Yuxia;DAI Lili;XUE Xiaojing;YU Yue;ZHANG Jianli;DING Xinhua;ZHONG Weiran;CHU Zhaohui;LIU Haifeng(State Key Laboratory of Crop Biology,College of Agronomy,Shandong Agricultural University,Taian 271018,China;Shandong Provin-cial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,College of Plant Protection,Shandong Agricultural University,Taian 271018,China;State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan 430072,China;Special Crops Institute,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;Shandong Pengbo Bio-technology,Taian 271018,China)

机构地区：[1]作物生物学国家重点实验室,山东农业大学农学院,泰安271018 [2]山东省蔬菜病虫生物学重点实验,山东农业大学植物保护学院,泰安271018 [3]杂交水稻国家重点实验室,武汉大学生命科学学院,武汉430072 [4]特色作物研究所,重庆市农业科学院,重庆401329 [5]山东蓬勃生物科技有限公司,泰安271018

出　　处：《植物病理学报》2024年第4期746-755,共10页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31801722);山东省重点研发计划(2019LYXZ007)。

摘　　要：由致病疫霉(Phytophthora infestans)引起的晚疫病严重危害番茄的产量和品质。番茄对晚疫病存在阶段性抗性,但具体抗性机制尚不清楚。本研究以番茄品种“小汤姆”为供试材料,发现该品种幼苗期(株龄4周)比成株期(株龄8周)更抗晚疫病。利用RNA测序和实时荧光定量PCR技术富集并验证了幼苗期番茄茉莉酸(Jasmonic acid,JA)生物合成途径中AOS1、AOS2和AOC等基因的转录水平显著高于成株期植株。植物激素检测结果也显示,番茄叶片中JA含量在幼苗期显著高于成株期。在本生烟叶片中瞬时表达AOS1、AOS2、AOC等基因均增强了烟草对晚疫病的抗性,表明这些JA合成相关基因的表达水平和植物对晚疫病的抗性水平之间呈正相关。体外喷施茉莉酸甲酯(Methyl Jasmonate,MeJA)能提高番茄植株对晚疫病的抗性,而喷施JA合成抑制剂DIECA则使番茄更感晚疫病,进一步证明了JA正向调控番茄对晚疫病的抗性。上述结果表明,JA合成相关基因的差异表达在番茄对晚疫病的阶段性抗性中发挥着重要作用。本研究为利用阶段性抗性防治番茄晚疫病提供了重要依据。Late blight caused by Phytophthora infestans seriously affects the yield and quality of tomato.Previous research found that tomato plants contain age-related resistance(ARR)to P.infestans,but the underlying mechanisms are still unclear.Here,we used tomato variety‘Micro Tom'as the tested material and found that younger(4-week-old)plants are more resistant while older(8-week-old)plants are more susceptible to late blight.Through RNA sequencing and real-time quantitative PCR(qPCR)analysis,we observed that the transcription levels of genes involved in jasmonic acid(JA)synthesis,such as AOS1,AOS2 and AOC,are higher in 4-week-old plants than those in 8-week-old plants.We further examined the levels of several phytohormones and found that the concentration of JA in 4-week-old plants is significantly higher than that in 8-week-old plants.Transient expression of AOS1,AOS2 or AOC in tobacco leaves made them more resistant to late blight,suggesting that these JA biosynthetic genes can enhance tomato resistance to late blight.Tomato plants sprayed with MeJA were more resistant whereas tomato plants sprayed with JA synthesis inhibitor DIECA were more susceptible to late blight,suggesting that JA positively regulates tomato resistance to late blight.Thus,we provide evidence supporting a model in which genes involved in JA synthesis play important roles in the age-related resistance to late blight in tomato.Our results lay an important basis for using ARR to control tomato late blight.

关 键 词：番茄晚疫病 阶段性抗性 茉莉酸 AOS AOC 

分 类 号：S436[农业科学—农业昆虫与害虫防治]