水稻恢复系‘雅恢2115'含有抗稻瘟病基因Pib  

The elite rice restorer line‘Yahui2115’carries blast resistance gene Pib

作　　者：胡小红 李恒进 石军 李德强[1] 樊晶[1] 黄富[2] 王文明[1] HU Xiaohong;LI Hengjin;SHI Jun;LI Deqiang;FAN Jing;HUANG Fu;WANG Wenming(State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,China;College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China;Yibin Academy of Agricul-tural Sciences,Yibin 64460,China;Mianyang Academy of Agricultural Sciences/Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province,Mianyang 621023,China)

机构地区：[1]四川农业大学西南作物基因资源发掘与利用国家重点实验室,成都611130 [2]四川农业大学农学院,成都611130 [3]宜宾市农业科学院,宜宾644600 [4]绵阳市农业科学研究院/厅市共建作物特色资源创制及应用四川省重点实验室,绵阳621023

出　　处：《植物病理学报》2024年第4期769-776,共8页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(U19A2033、32301838);四川省青年科技创新研究团队(2022JDTD0023);四川省自然科学基金(2022NSFSC1715)。

摘　　要：稻瘟病对水稻生产具有毁灭性的危害。选育和利用抗病品种是控制稻瘟病最经济有效且环保的方法。明确抗病品系的抗病基因将有利于选育新的抗病品种及品种合理布局。‘雅恢2115是广谱高抗稻瘟病的优良恢复系,前期研究表明其中含有抗瘟基因Pi2。为了鉴定‘雅恢2115'中其他抗瘟基因,本研究利用对Pi2单基因系致病的稻瘟菌菌株M14Ga089和丽江新团黑谷/雅恢2115'的F2代群体进行抗瘟基因定位分析。结果发现F2代群体的抗/感比率符合3:1的显性单基因分离比,表明‘雅恢2115'对菌株M14Ga089的抗性由1个显性基因控制,将其命名为Pir2115-1(t)。通过极端隐性群体法将Pir2115-1(t)基因定位到第2号染色体RM6307和RM208之间,该区域包含一个已克隆的抗瘟基因Pib。基因表达分析发现在‘雅恢2115'中Pib受稻瘟菌侵染高度诱导表达,序列分析发现‘雅恢2115'中Pib编码的蛋白质序列与已报道的Pib完全一致,表明‘雅恢2115'中含有Pib。本研究结果将为‘雅恢2115'在育种中的运用及其组合的品种布局提供重要依据。Rice blast disease is destructive to rice production.Breeding and utilization of resistant varieties are the most economical,effective and environmentally friendly methods to control the rice blast.Identifying the resistance genes of resistant varieties will be beneficial to the selection of new resistant varieties and the distribution of resistant varieties.‘Yahui2115'is an elite rice restorer line with broad-spectrum resistance to rice blast.Previous studies have shown that‘Yahui2115'contains Pi2.In order to identify other rice blast resistance genes in'Yahui2115',we used the strain M14Ga089 virulent to the monogenic line harboring Pi2 to analyze the segregation of blast resistance in the F2 population of'LTH/Yahui2115'.The results showed that the resistance/susceptibility ratio was 3:1 in the F2 population,indicating that the resistance from‘Yahui2115'to strain M14Ga089 was controlled by one dominant gene,which was named Pir2115-1(t).Through extreme recessive population method,Pir2115-1(t)was mapped between SSR markers RM6307 and RM208 on chromosome 2,a locus that contains a known blast resistance gene,Pib.Gene expression analysis showed that Pib in‘Yahui2115'was highly induced by Magnaporthe oryzae infection,and sequence analysis showed that the protein sequence encoded by Pib in‘Yahui2115'was completely identical to the reported Pib,indicating that‘Yahui2115'carries Pib.This study provides the basis for the application of Yahui2115'in breeding and the variety distribution of its combinations.

关 键 词：水稻 恢复系 稻瘟病 抗病基因 

分 类 号：S432.1[农业科学—植物病理学]