粗叶榕茎枯病病原菌的分离与鉴定  

Isolation and identification of theepathogen causing stem blight of Ficus hirta

作　　者：贺春萍[1,2] 胡月 吴贺 梁艳琼 李锐[1] 林金晶[1,2] 陆英 吴伟怀[1] 易克贤[1,4] HE Chunping;HU Yue;WU He;LIANG Yanqiong;LI Rui;LIN Jinjing;LU Ying;WU Weihuai;YI Kexian(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical crops,Ministry of Agriculture,P.R.China/Key Laboratory of Tropical Agricultural Pest Monitoring and Control,Hainan Province,Haikou 57110l,China;College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences,Sanya 572025,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海口571101 [2]华中农业大学植物科学技术学院,武汉430070 [3]云南农业大学农学与生物技术学院,昆明650201 [4]中国热带农业科学院三亚研究院,三亚572025

出　　处：《植物病理学报》2024年第4期857-861,共5页Acta Phytopathologica Sinica

基　　金：海南省院士创新平台科研专项(YSPTZX202016);海南省自然科学基金项目(320RC692);国家天然橡胶产业技术体系病害防控岗位(CARS-33-BC1)。

摘　　要：粗叶榕(Ficus hirta Vahl),又名五指毛桃、五爪龙、五指牛奶、亚桠木(海南)、南芪、土黄芪等^([1]),主要产于云南、贵州、海南、广西、广东、福建等地,富含蛋白质、多糖及黄酮类等物质,以根部入药,具有益气、健脾、润肺、祛湿等功效^([2]),是华南地区著名的药食同源植物和药材,享有“广东人参”的美誉,深受人们喜爱。Ficus hirta(vahl)is a valuable medicinal plant as well as edible plants in southern China.In 2022,a new stem blight disease was observed to infect Ficus hirta(vahl)in Danzhou of Hainan province.The incidence of disease reached 47.5%in fields.In order to clarify the pathogen of Ficus hirta stem blight disease,ten isolates with the same morphology were isolated from blight tissue by using a conventional tissue separation method.MA2,a representative strain with excellent growth,was selected for pathogenicity test on Ficus hirta plants,observation of morphological characteristics,sequence analysis on ITS,TUB and TEF,and phylogenetic tree construction.The results showed that morphological characteristics of the isolate MA2 was similar to that of Lasiodiplodia pseudotheobromae.The isolate MA2 was clustered into one clade in phylogenetic tree with L.pseudotheobromae.The above results indicated that the stem blight of F.hirta was caused by L.pseudotheobromae.This is the first report of a stem blight disease on F.hirta in China was identified as L.pseudotheobromae.

关 键 词：茎枯病 土黄芪 粗叶榕 五指毛桃 药食同源植物 分离与鉴定 黄酮类 人参 

分 类 号：S432.44[农业科学—植物病理学]