侵染浙江省和山东省西瓜的西瓜银灰斑驳病毒的鉴定  

Identification of watermelon silver mottle virus on watermelon in Zhejiang and Shandong provinces

作　　者：吴会杰[1] 严蕾艳 王驰 孙建磊[4] 王崇启[4] 高超[4] 康保珊[1] 刘莉铭[1] 刘茜 古勤生[1] WU Huijie;YAN Leiyan;WANG Chi;SUN Jianlei;WANG Chongqi;GAO Chao;KANG Baoshan;LIU Liming;LIU Xi;GU Qinsheng(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;Institute of Vegetables,Ningbo Academy of Agricultur-al Sciences,Ningbo 315040,China;Wenling Agricultural,Rural and Water Resources Bureau,Wenling 317500,China;Institute of Vegetables Shandong Academy of Agriculture Sciences,Jinan 250131,China)

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]宁波市农业科学院蔬菜研究所,宁波315040 [3]温岭市农业农村和水利局,温岭317500 [4]山东省农业科学院蔬菜研究所,济南250100

出　　处：《植物病理学报》2024年第4期877-880,共4页Acta Phytopathologica Sinica

基　　金：国家现代农业产业技术体系(CARS-25-2023-G20);中国农业科学院科技创新工程(CAAS-ASTIP-2022-ZFRI-09)。

摘　　要：西瓜银斑驳病毒(watermelon silver mottle virus,WSMoV)是番茄斑萎病毒科(Tospoviridae)正番茄斑萎病毒属(Orthotospovirus)病毒。可危害番茄、辣椒、西瓜等茄科和葫芦科作物,引起褪绿轮纹、环斑、皱缩等症状,造成严重的经济损失。During the disease survey in spring of 2023,watermelon in Shandong province and Zhenjiang province displayed silver grey and irregular mottled symptoms on leaves.In order to identify the virus,ten samples including the leaves,stem and the young fruit were collected,and total RNA was extracted from each sample.The RT-PCR method was used to amplify the conserved partial N gene sequence encoded by watermelon silver mottle virus(WSMoV)S RNA,and the partial N gene was cloned and sequenced.Sequence analysis showed that the sequence identity of WSMoV-Zhejiang and WSMoV-Shandong isolates with WSMoV-Thailand SN858 and WSMoV-Yunnan Banna-2011 isolates was over 99.0%.Furthermore,phylogenetic analysis showed that the partial N gene clustered with the Thailand and Yunnan-Banna-201l isolates.The results showed that watermelon silver mottle virus(WSMoV)occurred in watermelon in Zhejiang and Shandong provinces.This is the first time the virus has infected watermelon in Zhejiang and Shandong provinces.

关 键 词：番茄斑萎病毒 葫芦科作物 褪绿 斑驳病毒 环斑 西瓜 

分 类 号：S432.44[农业科学—植物病理学]