Modification of plasma protein for bioprinting via photopolymerization  

作　　者：Wenbi Wu Yinchu Dong Haofan Liu Xuebing Jiang Li Li Yi Zhang Maling Gou 

机构地区：[1]Department of Biotherapy,Cancer Center and State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China [2]Chengdu OrganoidMed Medical Laboratory,West China Health Valley,Chengdu 610043,China

出　　处：《Chinese Chemical Letters》2024年第8期486-490,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation(No.32271468);Sichuan Science and Technology Program(No.2021JDTD0001);Natural Science Foundation of Sichuan Province(No.2022NSFSC1280);Post-Doctor Research Project,West China Hospital,Sichuan University(No.2023HXBH081);Sichuan Science and Technology Program(No.2021YFS0124)。

摘　　要：Bioprinting is emerging as an advanced tool in tissue engineering.However,there is still a lack of bioinks able to form hydrogels with desirable bioactivities that support positive cell behaviors.In this study,modified plasma proteins capable of forming hydrogels with multiple biological functions are developed as bioinks for digital light processing(DLP)printing.The Plasma-MA(BM)was synthesized via a one-pot method through the reaction between the fresh frozen plasma and methacrylic anhydride.The methacry-lated levels were observed to influence the physical properties of BM hydrogels including mechanical properties,swelling,and degradation.The photo-crosslinked BM hydrogels can sustainedly release vascu-lar endothelial growth factor(VEGF)and exhibit positive biological effects on cell adhesion and prolifer-ation,and cell functionality such as tube formation of human umbilical vein endothelial cells(HUVECs),and neurite elongation of rat pheochromocytoma cells(PC12).Meanwhile,BM hydrogels can also induce cell infiltration,modulate immune response,and promote angiogenesis in vivo.Moreover,the plasma bioinks can be used to fabricate customized scaffolds with complex structures through a DLP printing process.These findings implicate that the modified plasma with growth factor release is a promising candidate for bioprinting in autologous and personalized tissue engineering.

关 键 词：BIOPRINTING Bioink PHOTOPOLYMERIZATION PLASMA Growth factor 

分 类 号：O631[理学—高分子化学] R318[理学—化学]