不同品种梅花鹿成纤维细胞系分离培养与保种库的建立  

Isolation and Culture of Different Species of Sika Deer Fibroblast Cell Lines and Establishment of Seed Preservation Bank

作　　者：刘昕 韩志强 张淑莹 赵伟刚[3] 么乃全[1] 李健明[4] 赵全民[4] 杜锐[4] 徐超 LIU Xin;HAN Zhiqiang;ZHANG Shuying;ZHAO Weigang;YAO Naiquan;LI Jianming;ZHAO Quanmin;DU Rui;XU Chao(College of Animal Science and Technology/College of Veterinary Medicine,Jilin Agricultural University,Changchun 130118,China;Ginseng and Antler Products Testing Center,Ministry of Agriculture,Changchun 130118,China;Institute of Special Animal and Plant Sciences,CAAS,Changchun 130112,China;College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学动物科学技术学院/动物医学院,长春130118 [2]农业农村部参茸产品质量检验测试中心,长春130118 [3]中国农业科学院特产研究所,长春130112 [4]吉林农业大学中药材学院,长春130118

出　　处：《经济动物学报》2024年第3期173-177,共5页Journal of Economic Animal

基　　金：吉林省畜禽遗传资源开发利用及科技提质增效项目(202404-3);吉林省农业关键核心技术示范推广项目(JARS-2024-0801-07)。

摘　　要：为研究梅花鹿成纤维细胞的分离培养方法并建立保种库,通过酶消化培养法和组织块培养法对梅花鹿原代成纤维细胞的分离培养效率进行分析。结果表明:酶消化培养法对梅花鹿成纤维细胞分离培养效果较好,并通过原代培养、冷冻和复苏建立了梅花鹿成纤维细胞保种库。研究结果为梅花鹿种质资源保存、体细胞核移植和基因克隆研究奠定基础。In order to study the isolation and culture method of sika deer fibroblasts and establish a seed bank,this study analyzed and compared the efficiency of isolation and culture of sika deer primary fibroblasts by enzyme digestion culture method and tissue block culture method,and proved that the isolation and culture of sika deer fibroblasts had achieved better results by the enzyme digestion culture method,and a sika deer fibroblast seed bank has been established through primary culture,freezing and recovery.The research aims to lay the foundation for the preservation of sika deer germplasm resources,somatic cell nuclear transplantation and gene cloning research.

关 键 词：梅花鹿 成纤维细胞 酶消化培养法 组织块培养法 

分 类 号：S825[农业科学—畜牧学]