鹿茸干细胞对大鼠坐骨神经损伤的再生修复作用  

Effects of Antler Stem Cells on Regeneration and Repair of Sciatic Nerve Injury in Rats

作　　者：叶子豪 李吉萍 王东旭 汪珍 任晶 郭倩倩 李春义 YE Zihao;LI Jiping;WANG Dongxu;WANG Zhen;REN Jing;GUO Qianqian;LI Chunyi(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Institute of Antler Science and Product Technology,Changchun Sci-Tech University,Changchun 130600,China)

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]长春科技学院鹿茸科学与产品技术研究所,长春130600

出　　处：《经济动物学报》2024年第3期178-187,共10页Journal of Economic Animal

基　　金：吉林省自然科学基金项目(YDZJ202201ZYTS435);国家自然科学基金区域联合基金项目(U20A20403);吉林省发展和改革委员会项目(2023C040-9)。

摘　　要：为探讨鹿茸干细胞对大鼠坐骨神经损伤的修复作用,选择30只雄性SD大鼠,制备大鼠坐骨神经损伤模型,随机分为3组:鹿茸干细胞(ASC)移植组,大鼠骨髓间充质干细胞(rBMSC)移植组和生理盐水(对照组),每组各10只。干细胞移植组通过尾静脉注射1×10^(6)个细胞(重悬于生理盐水)进行治疗,对照组注射同等剂量的生理盐水。分别于治疗后第7,21天取材检测。行为学观察与足迹分析检测神经功能恢复,组织学染色评估大鼠坐骨神经病理学变化与患侧腓肠肌萎缩恢复情况,免疫荧光染色与RT-qPCR检测坐骨神经再生相关因子(NF200,c-Jun,Periaxin,MPZ与Krox20)的表达。术后3周,与对照组相比,干细胞移植组大鼠坐骨神经功能情况和神经再生情况均出现明显改善,其中ASC移植组效果更显著。坐骨神经功能指数(SFI)结果显示,术后第21天,ASC移植组SFI值显著优于rBMSC移植组与对照组;术后第21天,对照组的患侧腓肠肌的湿重比、肌纤维面积显著低于干细胞移植组;组织学染色结果显示,ASC移植组再生神经纤维数目显著增多,排列规则,NF200表达量显著升高;术后第7天,RT-qPCR结果显示,与对照组相比,ASC移植显著增强c-Jun、Periaxin、MPZ与Krox20基因表达。首次基于鹿茸神经能够周期性再生这一生物学现象,探究鹿茸干细胞对大鼠神经再生修复的作用,明确了ASC移植显著促进大鼠坐骨神经损伤后的再生修复及其运动功能恢复,效果优于rBMSC移植组。To investigate the repairing effect of antler stem cells on sciatic nerve injury in rats,30 male SD rats were prepared to create sciatic nerve injury model and randomly divided into three groups:antler stem cell(ASC)transplantation group,rat bone marrow mesenchymal stem cell(rBMSC)transplantation group and saline control group,with 10 rats in each group.The stem cell transplantation group was treated by injecting 1×10^(6) cells(suspended with normal saline)into the tail vein,while the control group was given the same dose of normal saline.Samples were collected at day 7 and day 21 after treatment.Behavioral observation and footprint analysis were conducted to detect the recovery of nerve function.Histological staining was used to evaluate the pathological changes of sciatic nerve and the recovery of gastranemius atrophy on the affected side.The expression levels of NF200,c-Jun,Periaxin,MPZ and Krox20 related to sciatic nerve regeneration were detected by immunofluorescence staining and RT-qPCR.The results showed at 3 weeks after surgery,sciatic nerve function and nerve regeneration were significantly improved in the stem cell transplantation group compared with those in the control group,and the effect was more significant in the ASC transplantation group.SFI data showed that at 3 weeks after surgery,the SFI value of ASC group was significantly better than that of rBMSC group and control group.At 21 days after surgery,the wetweight ratio and muscle fiber area of gastrocnemius of the affected side of the control group were significantly lower than those of the stem cell transplantation group,and the rBMSC transplantation group was significantly lower than that of the ASC transplantation group.Histological staining results showed that the number of regenerated nerve fibers in ASC transplantation group increased significantly,the arrangement was regular,and the expression level of NF200 increased significantly.At day 7 after surgery,RT-qPCR results showed that ASC transplantation significantly enhanced c-Jun,Periax

关 键 词：鹿茸 干细胞 坐骨神经损伤 再生修复作用 

分 类 号：S825[农业科学—畜牧学]