鹿茸干细胞条件培养基通过调控NF-κB通路改善小鼠银屑病样皮损  

Deer Antler Stem Cell-Derived Conditioned Medium Improves Psoriasis-Like Skin Lesions in Mice via Regulating the NF-κB Pathway

作　　者：任晶 王东旭 李吉萍 汪珍 朱爽 马超 李春义 张国坤 REN Jing;WANG Dongxu;LI Jiping;WANG Zhen;ZHU Shuang;MA Chao;LI Chunyi;ZHANG Guokun(Institute of Antler Science and Product Technology,Changchun Sci-Tech University,Changchun 130600,China;College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]长春科技学院鹿茸科学与产品技术研究所,长春130600 [2]吉林农业大学中药材学院,长春130118

出　　处：《经济动物学报》2024年第3期188-196,共9页Journal of Economic Animal

基　　金：吉林省科技发展计划项目(YDZJ202301ZYTS508,20240602094RC);国家自然科学基金项目(32300708,U20A20403)。

摘　　要：为探究鹿茸干细胞(AnSC)的分泌物,即AnSC条件培养基(AnSC-CM)对咪喹莫特诱导的小鼠银屑病样皮损的改善作用及其潜在作用机制。采用咪喹莫特乳膏涂抹构建银屑病小鼠模型,将40只小鼠随机分为模型组、骨髓间充质干细胞条件培养基(BMSC-CM)阳性治疗组、AnSC-CM治疗组和正常组,每组10只。形态学观察不同组小鼠皮损严重程度,分析各组小鼠皮损面积和严重程度指数(PASI),苏木素-伊红(HE)染色观察各组小鼠损伤皮肤的组织病理变化;免疫组化染色检测AnSC-CM对小鼠表皮细胞异常增殖及炎症的影响;Western blotting检测核转录因子(NF-κB)通路的激活情况。结果表明:与正常组相比,模型组小鼠出现了明显的银屑病病变,红斑评分、鳞屑评分、皮损厚评分、总PASI评分和表皮厚均极显著增加(P<0.01);与模型组相比,AnSC-CM组和BMSC-CM组皮损症状均减轻,红斑评分、鳞屑评分、皮损厚评分、总PASI评分和表皮厚均极显著降低(P<0.01),且AnSC-CM的效果优于BMSC-CM。进一步研究发现,AnSC-CM还抑制了表皮细胞异常增殖,且AnSC-CM组银屑病样皮损组织中KRT14、PCNA、吲哚胺2,3-双加氧酶(IDO)和CD163的表达量均极显著降低(P<0.01),且NF-κB信号通路相关蛋白(p65,p-p65,IKB,p-IKB)的表达水平均极显著降低(P<0.01)。AnSC-CM可能通过抑制NF-κB信号通路的激活,从而抑制炎症的发生,进而在缓解银屑病中发挥重要作用。The aim of this study was to investigate the effects and underlying mechanism of deer antler stem cell secretions and its conditioned medium(AnSC-CM)on imiquimod-induced psoriasislike skin lesions in mice.A psoriasis mouse model was created using imiquimod cream.40 mice were then divided into a model group,a positive treatment group using bone marrow mesenchymal stem cell-derived conditioned medium(BMSC-CM),an AnSC-CM treatment group,and a normal control group,10 mice in each group.The severity of skin lesions was observed morphologically,and the psoriasis area and severity index(PASI)of skin lesions were analyzed in each group of mice.Histopathological changes in the damaged skin in each group of mice were examined using HE staining.Immunohistochemical staining was performed to assess the effects of AnSC-CM on abnormal proliferation and inflammation of mouse epidermal cells.Western blotting was used to detect the activation of the nuclear transcription factor-κB(NF-κB)pathway.The results showed that compared with the normal group,the model group mice showed significant psoriasis lesions,and the erythema,scale,skin lesion thickness,total PASI score and epidermal thickness were all extremely significantly increased(P<0.01).Compared with model group,the symptoms of skin lesions in both AnSC-CM and BMSC-CM groups were reduced,and the erythema,scale,skin lesion thickness,total PASI score and epidermal thickness were all extremely significantly decreased(P<0.01),and the effect of AnSC-CM was better than that of BMSC-CM.Further research found that AnSC-CM also significantly inhibited abnormal proliferation of epidermal cells,and the expression levels of KRT14,PCNA,indoleamine 2,3-dioxygenase(IDO),and CD163 in psoriasis-like skin lesions of AnSC-CM group were extremely significantly reduced(P<0.01),and the expression levels of NF-κB signaling pathway related proteins(p65,p-p65,IKB,and p-IKB)were extremely significantly reduced(P<0.01).The results of this study showed that AnSC-CM may play a crucial role in alleviatin

关 键 词：鹿茸干细胞分泌物 条件培养基 银屑病样皮损 炎症 NF-ΚB信号通路 

分 类 号：S852[农业科学—基础兽医学]