褪黑激素对常温保存梅花鹿精子的影响  

Effect of Melatonin on Sperm Survival of Cervus nippon in Vitro at Room Temperature

作　　者：刘舒欣 姜涛 衣岩 张舒童 陈新源 李昌泽 吴旻[1,2] 刘琳 段景玲 卫功庆 LIU Shuxin;JIANG Tao;YI Yan;ZHANG Shutong;CHEN Xinyuan;LI Changze;WU Min;LIU Lin;DUAN Jingling;WEI Gongqing(College of Animal Science and Technology/College of Veterinary Medicine,Jilin Agricultural University,Changchun 130118,China;Secondary Laboratory of"Medicinal Animal Breeding",Jilin Administration of Traditional Chinese Medicine,Changchun 130118,China;Changchun Dongda Deer Industry Co.LTD.,Changchun 130615,China)

机构地区：[1]吉林农业大学动物科学技术学院/动物医学院,长春130118 [2]吉林省中医药管理局“药用动物养殖”二级实验室,长春130118 [3]长春市东大鹿业有限公司,长春130615

出　　处：《经济动物学报》2024年第3期220-225,共6页Journal of Economic Animal

基　　金：吉林省科技发展计划重点研发项目(20210204030YY)。

摘　　要：为研究褪黑激素对常温保存梅花鹿精子的影响,将梅花鹿鲜精液分为5组,空白对照组用常温保存稀释液稀释,4个试验组分别添加不同量的褪黑激素进行等倍数稀释,稀释后的精液中褪黑激素质量浓度分别为10,20,40,80 mg/L。在常温条件下,每4 h检测精子活力、精子活率、质膜完整率、顶体完整率和线粒体活性等指标,至精子全部死亡,并计算各组精子的存活时间和存活指数。结果表明:添加褪黑激素能够降低常温保存梅花鹿精子的存活时间和存活指数,且与添加量呈线性负相关关系。常温保存8 h内,褪黑激素为10,20 mg/L时,精子活力和精子活率均显著高于对照组和其他试验组(P<0.05),20 mg/L试验组效果最佳;12 h时,各试验组的精子活率显著低于对照组(P<0.05)。精子的质膜完整率随时间延长呈下降趋势,褪黑激素对精子质膜完整率无显著保护作用。褪黑激素的添加量为20 mg/L时,精子顶体完整率显著升高(P<0.05);常温保存12 h内,随着时间的延长,精子顶体完整率呈下降趋势,且试验组的顶体完整率总体高于对照组;褪黑激素为20 mg/L时,顶体完整率最高。梅花鹿精子线粒体活性先升高后降低;添加10,20,40 mg/L的褪黑激素,精子线粒体活性显著高于对照组(P<0.05)。添加褪黑激素对常温保存梅花鹿精子有正向作用,保存时间不宜超过8 h,适宜添加量为20 mg/L。To study the effect of melatonin on the preservation of sika deer sperm at room temperature,fresh sika deer semen was divided into 5 groups.The control group was diluted with room temperature preservation diluent,and the 4 experimental groups were diluted with different amount of melatonin at equal multiples.The mass concentrations of melatonin in the diluted semen were 10,20,40,and 80 mg/L,respectively.Under normal temperature conditions,sperm motility,sperm motility,plasma membrane integrity,acrosome integrity,mitochondrial activity,and other indicators were detected every 4 hours until all sperm died,and the survival time and survival index of each group of sperm were calculated.The results showed that the addition of melatonin could reduce the survival time and survival index of sika deer sperm stored at room temperature,and there was a linear negative correlation with the addition amount of melatonin.Within 8 hours of storage at room temperature,when melatonin concentrations were 10,20 mg/L,the sperm motility and motility were significantly higher than those of the control group and the other experimental groups(P<0.05),and the 20 mg/L experimental group had the best effect.At 12 hours,the sperm motility of each experimental group was significantly lower than that of the control group(P<0.05).The sperm plasma membrane integrity rate showed a decreasing trend with time,no protective effect of melatonin on sperm plasma membrane integrity was observed.The addition concentration of melatonin within 20 mg/L did not have a significant effect on the sperm acrosome integrity rate(P>0.05),but the addition concentration of 40,80 mg/L significantly reduced the acrosome integrity rate(P<0.05).Within 12 hours,with the prolongation of time,the sperm acrosome integrity rate showed a decreasing trend,and the acrosome integrity rate of the experimental group was generally higher than that of the control group.When concentration of melatonin was 20 mg/L,the acrosome integrity rate was the highest.Within 12 hours,the mitochond

关 键 词：褪黑激素 梅花鹿 精子体外存活 常温保存 

分 类 号：S865.4[农业科学—野生动物驯养]