梅花鹿和马鹿卵母细胞获取技术研究进展  

Progress of Oocyte Collection Techniques in Sika Deer(Cervus nippon)and Red Deer(Cervus elaphus,Wapiti)

作　　者：谢书宇 黄承俊 XIE Shuyu;HUANG Chengjun(The Development and Service Center of Animal Husbandry of Yongcheng,Shangqiu 476600,China;Service Center for Agricultural Development of Liaoning Province,Shenyang 110032,China)

机构地区：[1]永城市畜牧发展服务中心,商丘476600 [2]辽宁省农业发展服务中心,沈阳110032

出　　处：《经济动物学报》2024年第3期259-264,共6页Journal of Economic Animal

基　　金：辽宁省科学技术计划项目(2022JH1/10400015)。

摘　　要：梅花鹿和马鹿卵母细胞的获取主要在繁殖季节进行,繁殖季节是母鹿配种的重要时期。梅花鹿和马鹿卵母细胞的获取,需兼顾母鹿的发情与配种。常用的取卵方法有手术法取卵、非手术法取卵和离体卵巢取卵。手术法取卵可分为手术法吸卵、手术法冲卵和腹腔镜法采卵(L-OPU);非手术法取卵是经B超介导,通过直肠把握卵巢,经阴道壁穿刺,吸取卵巢中卵母细胞的活体采卵法(B-OPU)。通过对梅花鹿和马鹿卵母细胞的获取技术进行综述,以期为鹿卵母细胞的收集提供参考。The oocytes of sika deer and red deer are mainly harvested during the breeding season,which is an important period for female deer breeding.Therefore,the oocyte collection from sika deer and red deer should also take into account the propagation of female deer.The commonly used methods for oocyte retrieval include surgical egg retrieval,non-surgical oocyte retrieval,and ex vivo ovarian oocyte retrieval.Surgical oocyte retrieval can be divided into surgical suction,surgical flushing,and laparoscopic egg retrieval(L-OPU).Non surgical oocyte retrieval refers to the live oocyte retrieval method(B-OPU)mediated by ultrasound,which involves grasping the ovaries through the rectum and puncturing the vaginal wall to extract oocytes from the ovaries.In this paper,the oocyte collection techniques of sika deer and red deer were reviewed and analyzed,which can provide reference for the reasonable recovery of deer oocyte.

关 键 词：梅花鹿 马鹿 卵母细胞 活体采卵 

分 类 号：S852[农业科学—基础兽医学]