宁夏贺兰山岩羊小反刍兽疫疫情来源分析  

Epidemic source analysis of peste des petits ruminants in Pseudois nayaur in Helan Mountain,Ningxia,China

作　　者：尹才[1,2] 李知新 李林 邸静[1,2] 周海宁 张玉玲 白涛涛 王玉梅 赵源 林邱雄 吴亚文 马龙 李莉娟 王晓亮 曾巧英 YIN Cai;LI Zhixin;LI Lin;DI Jing;ZHOU Haining;ZHANG Yuling;BAI Taotao;WANG Yumei;ZHAO Yuan;LIN Qiuxiong;WU Yawen;MA Long;LI Lijuan;WANG Xiaoliang;ZENG Qiaoying(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Ningxia Hui Autonomous Center for Animal Disease Control and Prevention,Yinchuan 750011,China;School of Agriculture,Ningxia University,Yinchuan 750021,China;China Animal Health and Epidemiology Center,Qingdao 266033,China;Helan County Center for Animal Disease Control and Prevention,Helan 750200,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]宁夏回族自治区动物疾病预防控制中心,银川750011 [3]宁夏大学农学院,银川750021 [4]中国动物卫生与流行病学中心,青岛266033 [5]贺兰县动物疾病预防控制中心,贺兰750200

出　　处：《兽类学报》2024年第4期497-505,共9页Acta Theriologica Sinica

基　　金：宁夏青年拔尖人才“自治区学术技术带头人”后备人选人才项目;自治区动物疫病净化科技创新团队项目(2020CXTDLX05)。

摘　　要：2018—2023年,宁夏贺兰山国家级自然保护区连续发现多起野生岩羊(Pseudois nayaur)不明原因死亡,通过解剖采样、实时荧光RT‑PCR检测,证实为小反刍兽疫病毒(PPRV)核酸阳性。使用Vero细胞分离出了部分毒株,采用RT‑PCR技术对宁夏地区病死家养羊和野生动物病料组织扩增PPRV F基因的全长片段。使用DNASTAR Lasergene和MEGA X软件对岩羊PPRV F基因序列进行比对和分析,构建系统发育树分析其分子遗传特征。结果显示:宁夏地区不同年度家养羊和贺兰山岩羊感染的PPRV毒株F基因同源性均在99%以上,其中2023年毒株与2018年、2019年1月毒株同源性最高,为99.8%,与2014年、2016年宁夏家养羊PPR疫情毒株及China/XJYL/2013(KM091959.1)同源性为99.6%。遗传进化分析表明,宁夏野生岩羊及家养羊PPRV同源性较高,均属于基因Ⅳ系中亚分支。因此推测宁夏及周边省份流行的PPR疫情可能通过不同途径在贺兰山野生动物种群中扩散蔓延,需加强PPR等野生动物疫源疫病监测。From 2018 to 2023,wild Pseudois nayaur were found to have died of unknown causes continuously in Helan Mountain National Nature Reserve of Ningxia,which was confirmed to be positive for peste des petits ruminants virus(PPRV)nucleic acid through anatomical sampling and real-time fluorescent RT-PCR detection.Vero cells were used to isolate some strains of PPRV,and RT-PCR was used to amplify the full-length fragments of PPRV F gene from diseased domestic sheep and wild animal samples in Ningxia.DNASTAR Lasergene and MEGA X were used to compare and analyze the PPRV F gene sequence and construct a phylogenetic tree to analyze its molecular genetic characteristics.The results show:the homology of F gene of PPRV strains infected by domestic sheep and Pseudois nayaur of Helan Mountain in different years in Ningxia was above 99%,among which the 2023 strain had the highest homology of 99.8%with that in January 2018 and 2019,and 99.6%with the PPR epidemic strains in 2014 and 2016 in Ningxia and China/XJYL/2013(KM091959.1)of Xinjiang.Genetic analysis showed that the PPRV of wild Pseudois nayaur and domestic sheep in Ningxia belonged to the central Asian branch of geneⅣline.Therefore,it is speculated that the epidemic of PPR in Ningxia and neighboring provinces may have spread in the wild animals of Helan Mountain through different ways,and it is necessary to strengthen the surveillance of PPR and other wild animal diseases.

关 键 词：宁夏贺兰山 岩羊 小反刍兽疫 分子遗传特征 

分 类 号：Q938[生物学—微生物学]