肺结核患者外周血T细胞协同刺激分子表达和NK细胞比例  

The expression of T cell costimulatory molecules and the proportion of NK cells in peripheral blood of patients with pulmonary tuberculosis

作　　者：杨晨晨 如克亚木·阿不都沙拉木 徐茜 YANG Chenchen;RU Ke Yamu·Abudusalamu;XU Qian(School of Health and Nursing,Wuxi Taihu University,Wuxi 214064;Department of Tuberculosis,The Eighth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000;Department of Immunology,School of Basic Medical Sciences,Xinjiang Medical University,Urumqi 830011,China;Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases,School of Basic Medical Sciences,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]无锡太湖学院健康与护理学院,江苏无锡214064 [2]新疆医科大学第八附属医院结核一科,新疆乌鲁木齐830000 [3]新疆医科大学基础医学院免疫学教研室,新疆乌鲁木齐830011 [4]新疆医科大学基础医学院新疆地方病分子生物学重点实验室,新疆乌鲁木齐830011

出　　处：《细胞与分子免疫学杂志》2024年第7期629-635,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：省部共建中亚高发病成因与防治国家重点实验室开放课题(SKL-HIDCA-2018-8);江苏省青蓝工程资助项目(苏教师函〔2021〕11号);江苏省无锡市滨湖区“滨湖之光”人才计划(苏滨科人领〔2021〕2号)。

摘　　要：目的检测肺结核(PTB)患者外周血协同刺激分子CD28、CD152/细胞毒性T淋巴细胞相关蛋白4(CTLA4)、程序性死亡受体1(PD-1)和自然杀伤(NK)细胞的水平,探讨PTB患者T细胞亚群活化和NK细胞功能,以及T细胞协同刺激信号分子在PTB发病机制中的作用。方法募集32名PTB患者(PTB组)和同期健康体检人员15名(对照组)。流式细胞术检测外周血T淋巴细胞CD28、CD152的表达,采用受试者工作特征(ROC)曲线分析两者之间的关系,流式细胞术检测外周血调节性T细胞(Treg)表面分子PD-1的表达以及NK细胞的比例。结果与对照组相比,PTB组CD8^(+)CD28^(+)T细胞比例、CD8^(+)CD152^(+)T细胞比例显著偏低。ROC曲线显示,变量CD8^(+)CD152^(+)T细胞比例对PTB的预测能力有一定准确性(AUC=0.800,CI=0.664~0.936)。CD4^(+)CD28^(+)T细胞以及CD4^(+)CD152^(+)T细胞比例无预测价值。PTB患者的CD4^(+)CD28^(+)T细胞和CD8^(+)CD28^(+)T细胞具有正相关关系(r=0.563)。与对照组相比,PTB组患者NK细胞比例显著降低。结论PTB患者CD8^(+)CD152^(+)T细胞、CD8^(+)CD28^(+)T细胞、NK细胞比例显著降低。Objective To investigate the levels of costimulator molecules CD28,CD152/CTLA4,PD-1 and NK cells in peripheral blood of patients with pulmonary tuberculosis(PTB),and to explore the activation of T cell subsets and function of NK cell in PTB patients,as well as the role of T cell costimulatory signaling molecules in the pathogenesis of PTB.Methods Thirty-two PTB patients(PTB group)and 15 health examiners(control group)were recruited.The expression of CD28 and CD152 on peripheral blood T lymphocytes was detected by flow cytometry.The relationship between the two group was analyzed using receiver operating characteristic(ROC)curves.The expression of PD-1 on regulatory T cells(Tregs)and the proportion of NK cells in peripheral blood were detected by flow cytometry.Results Compared with the control group,the proportions of CD8^(+)CD28^(+)T cells and CD8^(+)CD152^(+)T cells were significantly lower in the PTB group.The ROC curve showed that the variable CD8^(+)CD152^(+)T cell proportion had some predictive value in PTB(AUC=0.800,CI=0.664-0.936).The proportions of CD4^(+)CD28^(+)T cells and CD4^(+)CD152^(+)T cells had no predictive value.There was a positive correlation between CD4^(+)CD28^(+)T cells and CD8^(+)CD28^(+)T cells in PTB group(r=0.563).Compared with the control group,the proportion of NK cells was significantly reduced in the PTB group.Conclusion The proportions of CD8^(+)CD152^(+)T cells,CD8^(+)CD28^(+)T cells,and NK cells significantly reduced in PTB patients.

关 键 词：肺结核 自然杀伤(NK)细胞 CD28 CD152 程序性死亡受体1(PD-1) 

分 类 号：R521[医药卫生—内科学] R392.12[医药卫生—临床医学]