基因编码的细胞器钙指示剂研究进展  

Genetically encoded calcium indicators for organelles

作　　者：谷文佳 钟梓铱 张涵涵 张晓嫣 王友军[1,2] GU WenJia;ZHONG ZiYi;ZHANG HanHan;ZHANG XiaoYan;WANG YouJun(Beijing Key Laboratory of Gene Resource and Molecular Development,College of Life Sciences,Beijing Normal University,Beijing 100875,China;Key Laboratory of Cell Proliferation and Regulation Biology,Ministry of Education,College of Life Sciences,Beijing Normal University,Beijing 100875,China)

机构地区：[1]北京师范大学生命科学学院生物系,抗性基因资源与分子发育北京市重点实验室,北京100875 [2]北京师范大学生命科学学院生物系,细胞增殖及调控生物学教育部重点实验室,北京100875

出　　处：《中国科学：生命科学》2024年第8期1386-1407,共22页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:91954205,92254301)资助。

摘　　要：真核细胞中的内质网、线粒体等含钙细胞器,是细胞钙信号系统的重要组成部分.揭示这些细胞器中的钙离子动态及稳态有着重要的生理和病理意义.与化学钙探针相比,细胞器特异性表达的基因编码的钙指示剂(genetically encoded Ca^(2+)indicator, GECI)的定位更准确,使用更简便,因而具有很广阔的应用前景.本综述系统梳理了现有的线粒体、内质网、高尔基体、细胞内囊泡以及溶酶体等几种细胞器GECI的研究进展.总结了现有工具的优缺点,展望有待进一步优化的方向,旨在为今后细胞器GECI的开发及优化提供助力.In eukaryotic cells, calcium (Ca^(2+)) signal plays a vital role in regulating a wide range of physiological and pathological processes.Endoplasmic reticulum (ER), mitochondria and other Ca^(2+) containing organelles help precisely control the spatiotemporal Ca^(2+)dynamics and Ca^(2+) homeostasis. Compared with chemical Ca^(2+) indicators, the genetically encoded Ca^(2+) indicators (GECIs) are moreconvenient to use, show better site-specific localization, enabling broad applications. In this review, we summarized recent advancesin the development of organellar GECIs, especially those for mitochondria, ER, Golgi apparatus, endosomes and lysosome. Wemainly focused on their limitations and possible future optimization strategies, aiming to provide hints to help accelerate thedevelopment of better organellar GECIs.

关 键 词：细胞器钙指示剂 钙成像 细胞器钙信号 

分 类 号：Q25[生物学—细胞生物学]