Cr-金属有机框架/电化学还原氧化石墨烯/Nafion修饰电极测定多巴胺  

Cr-metal-organic framework/electrochemically reduced graphene oxide/Nafion modified electrode for the determination of dopamine

作　　者：徐燕 崔宏 XU Yan;CUI Hong(School of Public Health,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学公共卫生学院,太原030001

出　　处：《分析试验室》2024年第8期1073-1078,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(81803286);山西省自然科学基金(202203021211233);山西省高等学校科技创新项目(2020L0190)资助。

摘　　要：采用水热合成法制备了基于Cr的金属有机框架(Cr-MOFs),并加入树枝状大分子聚酰胺胺(PAMAM)调控其合成,扫描电镜和X射线衍射表征证明Cr-MOFs呈八面体形貌的晶体结构。Cr-MOFs与氧化石墨烯(GO)混合后经超声得到Cr-MOFs/GO复合材料。电化学还原得到Cr-MOFs/电化学还原氧化石墨烯/Nafion(Cr-MOFs/ERGO/Nafion)修饰电极用于检测多巴胺(DA),与ERGO/Nafion和Cr-MOFs/ERGO/Nafion修饰电极相比,DA的电流信号大大提高。在0.4~10μmol/L浓度范围内,DA的氧化峰电流与浓度呈良好的线性关系,检出限为0.1μmol/L。该方法展现了良好的重现性、稳定性及对尿酸(UA)和抗坏血酸(AA)的抗干扰能力,成功应用于人体尿样中DA含量的测定,加标回收率为84.0%~94.0%。Cr-based metal-organic frameworks(Cr-MOFs)were prepared by hydrothermal synthesis,and the dendritic macromolecule polyamidoamine(PAMAM)was added to control the synthesis.Scanning electron microscopy(SEM)and X-ray diffraction(XRD)characterization demonstrated that Cr-MOFs showed an octahedral shape and crystal structure.Cr-MOFs/GO composites were synthesized by ultrasound after mixing Cr-MOFs with graphene oxide(CO).Compared with the electrochemical reduction of graphene oxide/Nafion/glassy carbon electrode(ERGO/Nafion/GCE)and Cr-MOFs/ERGO/Nafion/GCE,the current signal of dopamine(DA)was greatly improved.In the concentration range of 0.4-10μmol/L,the oxidation peak current of DA showed a good linear relationship with its concentration,and the detection limit was O.1μmol/L.The method showed good reproducibility,stability and anti-interference ability for UA and AA,and was successfully applied to the determination of DA in human urine samples.The recoveries ranged from 84.0%to 94.0%.

关 键 词：金属有机框架 氧化石墨烯 多巴胺 电化学检测 

分 类 号：R115[医药卫生—公共卫生与预防医学] O657.1[理学—分析化学]