微波辅助法合成氮锌掺杂碳点用于检测氯霉素  

Microwave-assisted synthesis of nitrogen and zinc-doped carbon dots for the detection of chloramphenicol

作　　者：尚睿航 原照然 那婉园 宋昊 刘鹏[1] 曹秋娥[1] 周川华 SHANG Ruihang;YUAN Zhaoran;NA Wanyuan;SONG Hao;LIU Peng;CAO Qiue;ZHOU Chuanhua(School of Chemical Science and Technology,Yunnan University,National Demonstration Center for Experimental Chemistry and Chemical Engineering Education(Yunnan University),Kunming 650091,China)

机构地区：[1]云南大学化学科学与工程学院,化学化工国家级教学示范中心(云南大学),昆明650091

出　　处：《分析试验室》2024年第8期1146-1152,共7页Chinese Journal of Analysis Laboratory

基　　金：云南大学本科生科研项目(202205127)资助。

摘　　要：采用微波辅助法,以柠檬酸和尿素为前驱体,一水硫酸锌为掺杂剂,一步合成氮、锌掺杂碳点(N,Zn-CDs)。该碳点最大激发波长为370 nm,最大发射波长为439 nm,绝对荧光量子产率为22.67%。利用氯霉素(CAP)能使N,Zn-CDs荧光猝灭的特性,建立了一种检测CAP的荧光分析方法。在最优条件下,N,Zn-CDs的荧光强度与CAP浓度在7.0~2800.0μmol/L范围内呈良好的线性关系,方法检出限为5.5μmol/L。该方法已被成功应用于氯霉素针剂中CAP的检测。Nitrogen and zinc-doped carbon dots(N,Zn-CDs)were synthesized by one-step microwave-assisted method by using citric acid and urea as precursors,and zinc sulfate monohydrate as dopant.The maximum excitation wavelength of the carbon dots is at 370 nm,the maximum emission wavelength is at 439 nm,and the absolute fluorescence quantum yield is 22.67%.A fluorescence analysis method for the detection of chloramphenicol(CAP)was established based on the property that CAP could quench N,Zn-CDs fluorescence.Under the optimal conditions,the fluorescence intensity of N,Zn-CDs exhibited a good linear relationship with the concentration of CAP in the range of 7.0-2800.0μmol/L,and the detection limit was 5.5μmol/L.The method has been successfully applied to the detection of CAP in chloramphenicol injection.

关 键 词：碳点 金属掺杂 微波辅助法 氯霉素 荧光检测 

分 类 号：O657.3[理学—分析化学]