桔梗皂苷D改善对乙酰氨基酚致L02细胞损伤的作用及分子机制  

Protective Effect of Platycodin D on Acetaminophen Induced L02 Cell Injury and Its Molecular Mechanism

作　　者：张若冰 王士杰 沈琼 李伟 王梓[1] ZHANG Ruobing;WANG Shijie;SHEN Qiong;LI Wei;WANG Zi(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;College of Traditional Chinese Medicine,Jilin University of Agricultural Science and Technology,Jilin 132101,China)

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林农业科技学院中药学院,吉林132101

出　　处：《吉林农业大学学报》2024年第4期667-672,共6页Journal of Jilin Agricultural University

基　　金：吉林省教育厅“十三五”科学技术项目(JJKH20190946KJ);吉林省科技发展计划项目(20180201005YY)。

摘　　要：为研究桔梗皂苷(Platycodin D,PD)拮抗对乙酰氨基酚(Acetaminophen,APAP)致L02人源肝细胞损伤的保护作用与分子机制。建立L02肝细胞损伤的体外模型,筛选PD最佳给药剂量及时间,试剂盒测定细胞中谷胱甘肽(GSH)的含量,利用免疫荧光和Western Blot技术检测CYP2E1,iNOS,Nrf2,HO-1,Bax,Bcl-2,Caspase 3等蛋白的表达水平。PD在0.25,0.5,1μmol/L剂量时改善APAP引起的细胞活力,增加CYP2E1和iNOS的荧光强度(P <0.05或P <0.01),抑制过量GSH消耗,上调Nrf2依赖性抗氧化酶,Bcl-2及下调Bax、Caspase 3的表达(P <0.05或P <0.01)。PD可能部分通过调控Nrf2/HO-1信号通路发挥拮抗APAP致L02细胞损伤作用。To investigate the protective effect and molecular mechanism of platycodin D(PD)against acetaminophen(APAP)-induced injury of L02 human hepatocytes.We established the hepatocyte injury model of L02 in vitro,screened the optimal dose and time of PD administration,determined the content of glutathione(GSH)in the cell with a kit,and detected the expression levels of CYP2E1,iNOS,Nrf2,HO-1,Bax,Bcl-2 and Caspase-3 by immunofluorescence and Western blot.PD amelio⁃rated APAP induced cell viability in the dose range of 0.25,0.5 and 1μmol/L,increased the fluores⁃cence intensity of CYP2E1 and iNOS(P<0.05 or P<0.01),inhibited excessive GSH consumption,upregulated Nrf2 dependent antioxidant enzyme,Bcl-2 and down-regulated the expression of Bax and Caspase 3(P<0.05 or P<0.01).PD may antagonize the damage of L02 cells induced by APAP partly by regulating Nrf2/HO-1 signaling pathway.

关 键 词：桔梗皂苷D 对乙酰氨基酚 L02细胞 抗氧化作用 

分 类 号：S567.23[农业科学—中草药栽培]