不同年龄患儿不同类型标本的人巨细胞病毒-DNA检出率分析  

The detection rate of human cytomegalovirus⁃DNA in different types of specimens from children of different ages

作　　者：谢昕 任益慧 史利欢 范朋凯 陈静[1] XIE Xin;REN Yihui;SHI Lihuan;FAN Pengkai;CHEN Jing(Key Laboratory of Pediatric Hematology of Henan Province,Children's Hospital of Zhengzhou University,Zhengzhou,Henan 451161,China;The Neonatal Screening Center of Henan Province,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 451161,China)

机构地区：[1]郑州大学附属儿童医院河南省小儿血液医学重点实验室,河南郑州451161 [2]郑州大学第三附属医院河南省新生儿疾病筛查中心,河南郑州451161

出　　处：《热带医学杂志》2024年第7期989-993,共5页Journal of Tropical Medicine

基　　金：河南省医学科技攻关项目(LHGJ20200616)。

摘　　要：目的探讨血浆、尿液和母乳的人类巨细胞病毒(HCMV)⁃DNA在不同年龄段患儿的检出情况,为HCMV相关疾病提供诊疗依据。方法选择2016年1月-2021年12月收集于郑州大学附属儿童医院采用荧光定量PCR方法进行HCMV⁃DNA检测的临床标本共81641份,对不同年龄段患儿不同类型标本的HCMV⁃DNA以及患儿母亲母乳HCMV⁃DNA检出率进行回顾性分析。结果患儿血浆、尿液和母乳的HCMV⁃DNA阳性率分别为11.40%(8588/75302)、35.34%(1356/3837)、61.93%(1550/2503),血浆与尿液、母乳的HCMV⁃DNA阳性率差异均有统计学意义(χ^(2)=1903.85、5459.36,P均<0.001),尿液与母乳的HCMV⁃DNA阳性率差异有统计学意义(χ^(2)=431.95,P<0.001)。不同性别患儿血浆和尿液HCMV⁃DNA检出率差异均无统计学意义(χ^(2)=0.03、2.07,P均>0.05)。不同年龄组间比较,血浆和尿液HCMV⁃DNA阳性率差异均有统计学意义(χ^(2)=7466.71、1062.20,P均<0.001)。Bonferroni法对不同组间两两比较,不同年龄组间血浆HCMV⁃DNA阳性率,除≤21 d和>6个月~1岁组间、>21 d~3个月和>6个月~1岁组比较差异无统计学意义外,其余各组间血浆HCMV⁃DNA阳性率两两比较差异均有统计学意义(P均<0.05);不同年龄组间尿液HCMV⁃DNA阳性率,除≤21 d和其余各年龄组间、>21 d~3个月与>3~6个月、>6个月~1岁和>1~3岁组比较差异有统计学意义外,其余各年龄组间尿液HCMV⁃DNA阳性率两两比较差异均无统计学意义(P均>0.05)。进一步进行Spearman等级相关分析得出,患儿血浆HCMV⁃DNA阳性率与尿液HCMV⁃DNA阳性率成正相关(r=0.50,P<0.001);母乳HCMV⁃DNA阳性率与血浆、尿液HCMV⁃DNA阳性率无相关性(r=-0.17、0.04,P均>0.05)。结论不同年龄组患儿不同标本类型HCMV⁃DNA检出率不同,母乳HCMV⁃DNA检出率高于血浆和尿液,对HCMV感染患儿进行DNA检测时,应结合患儿年龄、生理特性,优先选择检出率高的标本类型,以此来提高HCMV⁃DNA的检出率。Objective To investigate the detection of human cytomegalovirus(HCMV)⁃DNA in plasma,urine and breast milk in children of different ages.Methods A total of 81641 clinical specimens collected from January 2016 to December 2021 at the Children's Hospital of Zhengzhou University for HCMV⁃DNA testing by fluorescent quantitative PCR were selected,and the detection rates of HCMV⁃DNA in different types of specimens from children of different age groups and breast milk of mothers of children were retrospectively analyzed.Results The HCMV⁃DNA positivity rates of plasma,urine and breast milk were 11.40%(8588/75302),35.34%(1356/3837)and 61.93%(1550/2503),respectively,and the differences between the positive rates of plasma and urine and breast milk(χ^(2)=1903.85,5459.36;all P<0.001)were statistically significant,and the differences between the positive rates of urine and breast milk were statistically significant(χ^(2)=431.95,P<0.001).The differences in the detection rates of HCMV⁃DNA in plasma and urine among children of different sexes were not statistically significant(χ^(2)=0.03,2.07;all P>0.05).The positive rates of HCMV⁃DNA in plasma and urine were significantly different among different age groups(χ^(2)=7466.71,1062.20;all P<0.001).Bonferroni method was used to compare the positive rates of plasma HCMV⁃DNA in different age groups;there was no significant difference between≤21 d and>6 month~1 year,>21 d~3 month and>6 month~1 year(P>0.05).The pair⁃to⁃pair comparison of plasma HCMV⁃DNA positive rate in other groups was statistically significant(P<0.05).The positive rate of urine HCMV⁃DNA in different age groups was statistically significant between≤21 d and other age groups,between>21 d~3 month and>3~≤6 month,>6 month~1 year and>1~3 years(P<0.05).There was no statistical significance in the positive rate of urine HCMV⁃DNA among other age groups(all P>0.05).Further Spearman rank correlation analysis yielded a positive correlation between plasma HCMV⁃DNA positivity and urine HCMV⁃DNA p

关 键 词：人巨细胞病毒 血浆 尿液 乳汁 

分 类 号：R511[医药卫生—内科学]