线扫式拉曼光谱仪的研制与生物医学应用进展  

Progress in Line-Scanning Raman Spectroscopy Developments and Applications in Biomedicine

作　　者：李逸凡 李小强 齐向东[1] 胡慧杰 宋一之[1,2] LI Yifan;LI Xiaoqiang;QI Xiangdong;HU Huijie;SONG Yizhi(Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Jiangsu Suzhou,215163;School of Biomedical Engineering(Suzhou),Division of Life Sciences and Medicine,University of Science and Technology of China,Anhui Hefei 230026)

机构地区：[1]中国科学院苏州生物医学工程技术研究所,江苏苏州215613 [2]中国科学技术大学生物医学工程学院(苏州),安徽合肥230026

出　　处：《光散射学报》2024年第3期320-329,共10页The Journal of Light Scattering

基　　金：国家重点研发计划(2022YFC2403300);国家自然科学基金(32170173);江苏省卓越博士后计划(2023ZB312)。

摘　　要：拉曼光谱技术是一种无损、高灵敏的分子成分检测技术,近年来在众多领域得到广泛应用,但由于逐点扫描的观测方式耗时较长,限制了在大面积样品拉曼扫描和成像中的应用。线扫式拉曼光谱通过线形激发光照明、同步采集线形样本区域拉曼信号的方式,相对于点扫式拉曼光谱仪成像速率获得了若干数量级的提升,从而使大面积、大数量样本具有时效性的拉曼光谱观测成为可能。本文对线扫式拉曼光谱仪的原理、搭建方式、系统结构和特点做了简要介绍,综述了其在细胞、组织观测等方面的应用进展,并对线扫式拉曼光谱性能改进及功能拓展技术进行了展望。Raman spectroscopy is a non-destructive and highly sensitive molecular component detection technique that has been widely applied in many fields in recent years.However,its applications in large scale example Raman imaging are limited due to the time-consuming procedure of point-scanning.Line-scanning Raman spectroscopy achieves several orders of magnitude improvements in imaging speed compared to point-scanning Raman spectroscopy by using line-shaped illumination light and synchronous collection of Raman signals from line-shaped sample areas,enabling timeliness Raman imaging of large scale and quantities of samples.This article provides a brief introduction to the principle,configuration and characteristics of line-scanning Raman spectrometer,summarizes its application progress mainly in cell and tissue observation,and makes a prospection on improving the performance and expanding functions of line-scanning Raman spectroscopy.

关 键 词：拉曼光谱 线扫 细胞成像 生物医学 

分 类 号：O43[机械工程—光学工程] O65[理学—光学]