受激拉曼显微技术及其在免染色组织病理学的应用  

Stimulated Raman microscopy and its applicationin stain-free histopathology

作　　者：马丽阳 罗宽 李佳颖 刘至杰 季敏标 MA Liyang;LUO Kuan;LI Jiaying;LIU Zhijie;JI Minbiao(State Key Laboratory of Surface Physics and Department of Physics of Fudan university,Shanghai,200433;Human Phenome Institute of Fudan university,Shanghai,200433;Academy for Engineering and Technology of Fudan university,Shanghai,200433)

机构地区：[1]复旦大学物理学系应用表面物理国家重点实验室,上海200433 [2]复旦大学人类表型组研究院,上海200433 [3]复旦大学工程与应用技术研究院,上海200433

出　　处：《光散射学报》2024年第3期343-362,共20页The Journal of Light Scattering

基　　金：科技部国家重点研发计划“工程科学与综合交叉”专项(2021YFF0502902);国家自然科学基金(61975033)。

摘　　要：近年来,许多基于光与物质相互作用的无标记光学成像方法被逐渐开发并在越来越多的领域发挥着重要作用。受激拉曼显微镜作为一种无标记振动成像工具,具有对组织学诊断最相关的主要生物分子的高分辨率化学特异性成像优势。在本文中,我们主要关注受激拉曼显微镜的技术发展和它在无染色和无切片快速组织成像中应用。在介绍光学原理和技术细节后,展示受激拉曼组织学在各种类型人类疾病中的应用。进一步结合深度学习算法,总结了对新鲜手术组织、胃镜活检和穿刺活检的案例研究,以展示人工智能辅助受激拉曼组织学在各种应用场景中的潜力。Recently,many label-free optical imaging methods based on the interaction between light and matter have been gradually developed and play an important role in more and more fields.Stimulated Raman microscopy,as an label-free vibrational imaging tool,has the advantage of high-resolution chemical specific imaging of the main biomolecules most relevant for histological diagnosis.In this article,we mainly focus on the technological development of stimulated Raman microscopy and its application in fast tissue imaging without staining or sectioning.After introducing optical principles and technical details,we demonstrated the application of stimulated Raman histology in various types of human diseases.Further combining deep learning algorithms,case studies on fresh surgical tissue,gastroscopy biopsy,and core needle biopsy were summarized to demonstrate the potential of artificial intelligence assisted stimulated Raman histology in various application.

关 键 词：非线性光学成像 受激拉曼显微镜 免染色组织病理学 

分 类 号：O437.3[机械工程—光学工程] Q-336[理学—光学] R602[理学—物理]