刺芒柄花素对白细胞介素1β诱导软骨细胞损伤影响的机制  

Mechanism underlying the effect of formononetin on interleukin-1beta-induced chondrocyte injury

作　　者：单继新 叶锐彬[1] 巨少华[1] 王强[1] Shan Jixin;Ye Ruibin;Ju Shaohua;Wang Qiang(Department of Bonesetting,Affiliated Sport Hospital of Chengdu Sport University,Chengdou 610041,Sichuan Province,China)

机构地区：[1]成都体育学院附属体育医院正骨科,四川省成都市610041

出　　处：《中国组织工程研究》2025年第12期2484-2491,共8页Chinese Journal of Tissue Engineering Research

基　　金：四川省中医药管理局科学技术研究专项课题项目(2023MS271),项目负责人:王强;四川省自然科学基金项目(2023NSFC1803),项目负责人:巨少华。

摘　　要：背景:刺芒柄花素是一种异黄酮类化合物,广泛存在于红车轴草、黄芪、鸡血藤中,具有抑制氧化应激、炎症因子释放及细胞凋亡的作用。目的:探讨刺芒柄花素对白细胞介素1β诱导软骨细胞损伤的影响及机制。方法:①收集骨关节炎患者及单纯半月板损伤患者的软骨组织,采用实时定量PCR检测miR-135b-5p表达。②体外培养人软骨细胞,分9组培养:正常对照组不进行任何处理,培养48 h;白细胞介素1β组加入白细胞介素1β处理48 h;白细胞介素1β+刺芒柄花素低浓度组加入25μmol/L刺芒柄花素处理24 h后加入白细胞介素1β处理48 h;白细胞介素1β+刺芒柄花素中浓度组加入50μmol/L刺芒柄花素处理24 h后加入白细胞介素1β处理48 h;白细胞介素1β+刺芒柄花素高浓度组加入100μmol/L刺芒柄花素处理24 h后加入白细胞介素1β处理48 h;白细胞介素1β+miR NC组转染miR NC 6 h后加入白细胞介素1β处理48 h;白细胞介素1β+miR-135b-5p组转染miR-135b-5p模拟物6 h后加入白细胞介素1β处理48 h;白细胞介素1β+刺芒柄花素高浓度+anti-miR-NC组转染anti-miR-NC 6 h后加入100μmol/L刺芒柄花素处理24 h,再加入白细胞介素1β处理48 h;白细胞介素1β+刺芒柄花素高浓度+anti-miR-135b-5p组转染anti-miR-135b-5p 6 h后加入100μmol/L刺芒柄花素处理24 h,再加入白细胞介素1β处理48 h。处理结束后进行相关检测。结果与结论:①骨关节炎患者软骨组织中miR-135b-5p表达低于单纯半月板损伤患者(P<0.05)。②与正常对照组比较,白细胞介素1β组软骨细胞miR-135b-5p表达、增殖活力、超氧化物歧化酶活性、Ⅱ型胶原蛋白、Bcl-2蛋白表达均降低(P<0.05),细胞凋亡率、乳酸脱氢酶活性、丙二醛水平、促炎因子水平、基质金属蛋白酶13蛋白、Bax蛋白表达均升高(P<0.05);刺芒柄花素可抑制白细胞介素1β对软骨细胞造成的损伤,并且呈现浓度依赖性。转染miR-135b-5p模拟物可�BACKGROUND:Formononetin is an isoflavonoid compound widely found in red clover,astragalus,and chickweed,which has the ability to inhibit oxidative stress,inflammatory factor release,and apoptosis.OBJECTIVE:To investigate the effect of formononetin on interleukin-1β-induced chondrocyte injury and its mechanism.METHODS:(1)Cartilage tissues from patients with osteoarthritis and patients with simple meniscus injury were collected,and real-time quantitative PCR was used to detect miR-135b-5p expression.(2)Human chondrocytes were cultured in vitro,and then divided them into nine groups:cells in normal control group were cultured for 48 hours with no treatment;cells in interleukin-1βgroup were treated with interleukin-1βfor 48 hours;cells in interleukin-1β+low-dose formononetin group were treated with 25μmol/L formononetin for 24 hours followed by treatment with interleukin-1βfor 48 hours;cells in interleukin-1β+middle-dose formononetin group were treated with 50μmol/L formononetin for 24 hours followed by treatment with interleukin-1βfor 48 hours;cells in interleukin-1β+high-dose formononetin group were treated with 100μmol/L formononetin for 24 hours followed by treatment with interleukin-1βfor 48 hours;cells in interleukin-1β+miR NC group were treated with miR NC for 6 hours followed by treatment with interleukin-1βfor 48 hours;cells in interleukin-1β+miR-135b-5p group were treated with miR-135b-5p mimics for 6 hours followed by treatment with interleukin-1βfor 48 hours;cells in interleukin-1β+high-dose formononetin+anti-miR-NC group were treated with anti-miR-NC for 6 hours,then treated with 100μmol/L formononetin for 24 hours,and finally treated with interleukin-1βfor 48 hours;cells in interleukin-1β+high-dose formononetin+anti-miR-135b-5p group were treated with anti-miR-135b-5p for 6 hours,then treated with 100μmol/L formononetin for 24 hours,and finally treated with interleukin-1βfor 48 hours.Relevant tests are performed after treatment.RESULTS AND CONCLUSION:The expression level of miR-135

关 键 词：刺芒柄花素 miR-135b-5p 软骨细胞 氧化应激 炎症 细胞增殖 细胞凋亡 

分 类 号：R453[医药卫生—治疗学] R319[医药卫生—临床医学] R684.3