中国对虾PcABCG5基因在高碳酸盐碱度胁迫下的表达模式分析  

Analysis of Expression Patterns of PcABCG5 Gene in Penaeus chinensis Under Saline-Alkali Stress

作　　者：李亚伦 何玉英[2] 王琼 管晨惠 周玉洁 李朝霞[1] LI Yalun;HE Yuying;WANG Qiong;GUAN Chenhui;ZHOU Yujie;LI Zhaoxia(College of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266237,China;Key Laboratory of Marine Fisheries and Sustainable Development,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116000,China)

机构地区：[1]青岛农业大学海洋科学与工程学院,山东青岛266237 [2]中国水产科学研究院黄海水产研究所、农业农村部海洋渔业与可持续发展重点实验室,山东青岛266071 [3]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《渔业科学进展》2024年第5期165-173,共9页Progress in Fishery Sciences

基　　金：国家虾蟹产业技术体系(CARS-48);国家自然科学基金(31772842);中国水产科学研究院基本科研业务费(2020TD46);中央提前下达渔业成品油价格改革财政补贴项目(326-0501-YZN-Z4HB)共同资助。

摘　　要：为探究急性盐碱胁迫条件下中国对虾(Penaeus chinensis)ABC转运蛋白(ATP-binding cassette transporter)G5基因的表达特征,本研究应用RACE克隆技术获得中国对虾ABCG5基因,并将其命名为PcABCG5。该基因cDNA全长为2718 bp,开放阅读框为1923 bp,共编码640个氨基酸,预测的蛋白分子量为71.39 kDa,理论等电点为9.12,预测基因位于内质网上,为疏水性蛋白,包含1个NBD和1个TMD结构域,不包含信号肽。同源性与系统进化分析结果显示,PcABCG5转运蛋白保守性强,与斑节对虾(Penaeus monodon)ABCG5蛋白同源性最高,达98.91%。组织特异性分析结果显示,PcABCG5在各组织中均有表达,鳃中表达水平最高(P<0.05),眼柄中最低(P<0.05)。在盐碱胁迫下,PcABCG5在鳃组织中的表达量呈先升高后下降的趋势,胁迫6 h时达到最高值(P<0.05),为对照组的3.12倍;利用dsRNA干扰实验对该基因进行敲降,沉默PcABCG5基因会使盐碱胁迫下的对虾死亡率高达60%,比对照组高40%(P<0.05),说明该基因的表达量越高,对中国对虾的存活越有利。本研究中受盐碱胁迫的中国对虾PcABCG5表达量高,可能是由于鳃在直接接触盐碱水环境后,通过PcABCG5介导的物质转运启动渗透压调节来应对渗透压失衡。Penaeus chinensis is one of the most economically important species in northern China.Strong market demand requires the expansion of aquaculture production capacity of P.chinensis.Saline-alkali water covers approximately 46 million hectares in China alone,primarily distributed in the northeast,northwest,and coastal areas.Low salinity,high carbonate alkalinity,high pH,and complex ionic composition are characteristics of such waters,which cause stress to aquatic animals by interfering with physiological homeostasis.High carbonate alkalinity can directly damage the gill tissue of crustaceans.The inhabitation of ion-exchange can result in alkalosis.The understanding of response mechanisms to saline-alkali stress in P.chinensis will contribute to the sustainable development of the shrimp industry.ATP-binding cassette(ABC)transporters are one of the most prominent families of transmembrane proteins.ABC transporters can transport molecules,such as inorganic ions,sugars,amino acids,lipids,peptides,specialized metabolites,and xenobiotic agents,across membranes by binding and hydrolyzing ATP(adenosine triphosphate).Members of the ABCG subfamily consist of a single ABC cassette in the amino terminal followed by six putative transmembrane domains,and thus,are referred to as half-sized ABC transporters.Members of this family play an important role in the efflux transport of cholesterol.The ABCG subfamily participates in signal transduction,antiviral defense,and antigen presentation through hormone transport and lipid metabolism,thus helping plants adapt to changing environments.Several members of this gene family show different expression patterns in P.chinensis when exposed to saline-alkali stress.Therefore,we aimed to explore the ABC functions in resistance to alkalosis in P.chinensis.In this study,ABCG5(GenBank accession number:OQ318160)was identified in P.chinensis and named PcABCG5.The full length of PcABCG5 was 1,923 base pairs,encoding 640 amino acids.The estimated molecular mass was 71.39 kDa,and the theoretical isoel

关 键 词：中国对虾 PcABCG5 盐碱胁迫 DSRNA 

分 类 号：S968.22[农业科学—水产养殖]