布氏乳杆菌和枯草芽孢杆菌对广西麻鸡繁殖性能和卵巢发育的影响  

Effect of dietary supplementation with Lactobacillus bruceiand Bacillus subtilis on reproductive performance and ovarian development of Guangxi Ma chickens

作　　者：陆素宁 朱敏[1,2] 黄嫣 马春霞 李常挺[3] 彭昊 陆阳清[1] 廖玉英[3] LU Su-ning;ZHU Min;HUANG Yan;MA Chun-xia;LI Chang-ting;PENG Hao;LU Yang-qing;LIAO Yu-ying

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西农业职业技术大学,广西南宁5300093 [3]广西壮族自治区兽医研究所,广西南宁530001

出　　处：《饲料研究》2024年第14期61-66,共6页Feed Research

基　　金：广西重点研发计划(项目编号:桂科AB21220005-4)。

摘　　要：试验旨在研究饲粮中添加布氏乳杆菌和枯草芽孢杆菌活菌液对产蛋高峰期广西麻鸡繁殖性能和卵巢发育的影响。选取健康状况良好、27周龄产蛋高峰期广西麻鸡480只,随机分为4组,每组3个重复,每个重复40只鸡。试验期9周。对照组饲喂基础饲粮,M1组在基础饲粮中添加布氏乳杆菌和枯草芽孢杆菌比例为1∶1的活菌液,L1组在基础饲粮中添加布氏乳杆菌活菌液,B1组在基础饲粮中添加枯草芽孢杆菌活菌液,益生菌制剂活菌数均为4.5×10^(8) CFU/mL。结果显示:与对照组相比,M1组第2~8周的产蛋率显著提高(P<0.05),平均产蛋率和合格种蛋率显著提高(P<0.05)。M1组血清雌激素(E)、孕酮(PROG)、促卵泡素(FSH)和促黄体素(LH)的生殖激素水平及卵巢雌激素受体(ER)mRNA、孕酮受体(PR)mRNA、促卵泡素受体(FSHR)mRNA和促黄体素受体(LHR)mRNA的相对表达量均显著提高(P<0.05)。研究表明,在饲粮中添加布氏乳杆菌和枯草芽孢杆菌混合菌液可改善产蛋高峰期广西麻鸡种鸡的繁殖性能,促进卵泡成熟和卵巢发育。The experiment aimed to study the effects of adding Lactobacillusbrucei and Bacillussubtilis live bacterial liquid in the diet on the reproductive performance and ovarian development of Guangxi Ma chickens during the peak egg-laying period.A total of 480 healthy Guangxi Ma chickens at 27 weeks of age during the peak egg-laying period were randomly divided into four groups,with three replicates per group,each replicate consisting of 40 chickens.The trial lasted for nine weeks.The control group was fed a basic diet,the M1 group was supplemented with a live bacterial liquid of Lactobacillus brucei and Bacillus subtilis in a 1∶1 ratio in the basic diet,the L1 group was supplemented with Lactobacillusbrucei live bacterial liquid in the basic diet,and the B1 group was supplemented with Bacillussubtilislive bacterial liquid in the basic diet,with the viable bacteria count of the probiotic preparation being 4.5×10^(8) CFU/mL.The results showed that compared with the control group,the egg-laying rate of the M1 group significantly increased(P<0.05)from the 2nd to the 8th week,and the average egg-laying rate and the rate of qualified breeding eggs significantly increased(P<0.05).The levels of reproductive hormones such as serum E,PROG,FSH,and LH in the M1 group,as well as the relative expression levels of ovarian ERmRNA,PRmRNA,FSHRmRNA,and LHRmRNA,were all significantly increased(P<0.05).The study indicates that the addition of a mixed bacterial liquid of Lactobacillusbrucei and Bacillus subtilisin the diet can improve the reproductive performance of Guangxi Ma breeder chickens during the peak egg-laying period and promote the maturation of follicles and the development of the ovaries.

关 键 词：布氏乳杆菌 枯草芽孢杆菌 广西麻鸡 繁殖性能 卵巢发育 

分 类 号：S831[农业科学—畜牧学]