金佛手总黄酮的提取工艺及体外抗氧性测试  

Extraction Process and Antioxidant Test of Total Flavonoids from Jinhua Bergamot

作　　者：王晨霞[1] 黄东纬 Wang Chenxia;Huang Dongwei(Pharmaceutical College,Jinhua 321000,China)

机构地区：[1]金华职业技术学院,浙江金华321000

出　　处：《山东化工》2024年第15期4-7,共4页Shandong Chemical Industry

基　　金：浙江省金华市公益类科学技术研究计划项目(2021-4-312)。

摘　　要：主要研究金华佛手(以下简称金佛手)提取精油及多糖后残渣中总黄酮的乙醇回流超声法提取工艺条件,并对总黄酮提取物进行抗氧化测试。采用单因素试验得到最佳提取工艺为:乙醇浓度75%、温度70℃、提取时间120 min、料液比1∶30(g∶mL,下同)、超声60 min;在此条件下总黄酮提取率1.43%;对DPPH自由基及ABTS自由基清除率分别为46.5%和62.4%,表明金佛手黄酮具有一定的抗氧化性。This article investigates the ethanol and ultrasonic extraction conditions for total flavonoids in the residue left after the extraction of essential oil and polysaccharides from Jinhua bergamot and conducts antioxidant tests on the total flavonoid extracts.The optimal extraction process was determined through single-factor experiments as follows:75%ethanol concentration,70℃temperature,120 minutes extraction time,a solid-liquid ratio of 1∶30,and 60 minutes of ultrasonication.Under these conditions,the extraction yield of total flavonoids was 1.43%.The scavenging rates for DPPH and ABTS radicals were 46.5%and 62.4%.So the flavonoids from Jin Bergamot possess certain antioxidant properties.

关 键 词：金佛手 总黄酮 提取工艺 体外抗氧性 

分 类 号：TQ461[化学工程—制药化工]