安徽池州道地黄精中总黄酮的提取工艺优化和含量测定研究  

Optimization of Extraction Process and Determination of Total Flavonoids in Rhizoma Polygonati from Chizhou,Anhui Province

作　　者：陈文霞 胡双艺 陈纭[1] 杨芮 Chen Wenxia;Hu Shuangyi;Chen Yun;Yang Rui(School of Pharmacy,Anhui Medical University,Anhui,230032)

机构地区：[1]安徽医科大学药学院,安徽230032

出　　处：《当代化工研究》2024年第16期40-42,共3页Modern Chemical Research

基　　金：2021年安徽省自然科学基金青年项目“基于微流控纸芯片和化学发光功能化磁性材料的高灵敏度免疫分析法用于肺癌的早期诊断”(项目编号:2108085QH378);2022年安徽医科大学药学创新基金科研项目“磺基水杨酸苦参碱的合成及治疗类风湿关节炎的机制研究”(项目编号:YXCX202201)。

摘　　要：利用超声提取法对池州黄精中总黄酮进行提取,并对提取条件进行优化,基于硝酸铝-亚硝酸钠比色原理,采用紫外-可见分光光度法测定总黄酮含量。最优乙醇体积分数、料液比、超声时间和超声温度分别为80%、1:10、25 min和30℃,该条件下测得黄精样品中总黄酮含量为0.735 mg/g。结果表明优化后的总黄酮提取效率高,建立的紫外-可见分光光度含量测定法精密度高、重复性好,可为池州黄精资源开发提供可行性依据。The total flavonoids in rhizoma polygonati were extracted by ultrasonic method,and the extraction conditions were optimized.The content of total flavonoids was determined by aluminum nitrate-sodium nitrite colorimetric method.The optimal ethanol concentration,solid-liq-uid ratio,ultrasonic time and ultrasonic temperature were 80%,1:10,25 min and 30℃,respectively.Under these conditions,the content of total fla-vonoids in rhizoma polygonati was 0.735 mg/g.The results showed that the optimized extraction efficiency of total flavonoids was high,and the es-tablished UV-visible spectrophotometry method had high precision and good repeatability,which could provide a feasible basis for the development of rhizoma polygonati resources in Chizhou.

关 键 词：池州黄精 总黄酮 超声提取法 紫外-可见分光光度法 

分 类 号：R284[医药卫生—中药学]