基于UPLC-QTOF-HRMS的夏天无地上部分生物碱类成分研究  

Study on Alkaloid Constituents of Aboveground Part of Corydalis Decumbens Based on UPLC-QTOF-HRMS

作　　者：黄泰奇 周易翔 熊鑫 蔡嘉东 HUANG Tai-qi;ZHOU Yi-xiang;XIONG Xin;CAI Jia-dong(School of Medicine,Wuhan University of Science and Technology,Hubei Wuhan 430065;School of Life Science,Beijing University of Chinese Medicine,Beijing 100000;Wuxi AppTec(Suzhou)Co.,Ltd.,Jiangshu Suzhou 215104,China)

机构地区：[1]武汉科技大学医学院,湖北武汉430065 [2]北京中医药大学生命科学学院,北京100000 [3]苏州药明康德新药开发有限公司,江苏苏州215104

出　　处：《广州化工》2024年第16期85-89,共5页GuangZhou Chemical Industry

摘　　要：采用UPLC-QTOF-HRMS法探究夏天无地上部分的生物类成分,方法如下:柱温:40℃,进样量:2μL,流速:0.4 mL/min,流动相A:0.1%甲酸,B:乙腈(含0.1%甲酸)梯度洗脱,采用正离子模式,通过相关文献和数据库分析并鉴定生物碱类成分。UPLC-QTOF-HRMS法检测结果中共鉴定出26种生物碱。上述检测结果可对夏天无地上部分开发利用提供理论依据。Alkaloid constituents of aboveground part of Corydalis decumbens were studied by UPLC-QTOF-HRMS.The method of UPLC-QTOF-HRMS was as follows,column temperature of 40℃,injection volume of 2μL,volume flow rate of 0.4 mL/min,mobile phase of water(with 0.1%formic acid)(A)-acetonitrile(with 0.1%formic acid)(B),gradient elution,using positive ion mode,analyzing and identifying the alkaloid constituents via related literature and databases.A total of 26 alkaloids were identified by UPLC-QTOF-HRMS.The results could provide a theoretical datum for the development of aboveground part of Corydalis decumbens.

关 键 词：夏天无 地上部分 生物碱 UPLC-QTOF-HRMS 

分 类 号：R284.2[医药卫生—中药学]