水杨酸对甜糯玉米幼苗抗寒、抗盐生理影响分析  

Effects of Salicylic Acid on Resistance Physiology of Sweet Glutinous Corn Seedlings under Low Temperature and Salt Stress

作　　者：凌娜[1] 王淼 梅雨楠 刘伊娜 苏燕 杨梓仪 LING Na;WANG Miao;MEI Yunan;LIU Yina;SU Yan;YANG Ziyi(College of Architecture and Landscape Shangqiu University,Shangqiu 476000,China)

机构地区：[1]商丘学院风景园林与建筑学院,河南商丘476000

出　　处：《商丘职业技术学院学报》2024年第4期87-92,共6页JOURNAL OF SHANGQIU POLYTECHNIC

基　　金：2022年度河南省教育科学规划课题“专创融合视角下应用型高校创新创业教育范式研究”(2022YB0478)。

摘　　要：为探究低温和盐胁迫下水杨酸对甜糯玉米幼苗抗性生理的影响,以甜糯玉米182为试验材料,通过外源叶面喷施水杨酸(SA),测定幼苗的生长特性、渗透调节物质、丙二醛(MDA)含量、过氧化氢酶(CAT)和过氧化物酶(POD)活性,从而明确水杨酸对甜糯玉米幼苗抗寒及抗盐生理的影响.结果表明:3℃低温和100 mmol/L NaCl处理使甜糯玉米幼苗的正常生长受到显著抑制,经不同浓度水杨酸喷施均能有效缓解低温胁迫和盐胁迫对幼苗造成的不利影响,其中以40 mg/L SA处理效果最佳.喷施40 mg/L水杨酸,使玉米幼苗株高、干物质积累、叶片相对含水量、可溶性糖和可溶性蛋白含量都出现不同程度的增加,MDA含量降低,抗氧化物酶CAT和POD活性增强.因此推测,通过叶面喷施水杨酸可能加强了胁迫条件下植株的渗透调节能力,增强了抗氧化酶的活性,减少了膜的损伤程度,综合提高了甜糯玉米幼苗的抗寒与抗盐能力.To investigate the resistance of maize seedlings treated with salicylic acid(SA)under low temperature and salt stress,the“sweet glutinous 182”maize was used as the experimental material,and its growth characteristics,the osmoregulation substances content,the Malondialdehyde(MDA)content,and the activities of Catalase(CAT)and Peroxidase(POD)enzymes between different treatment of seedlings were measured through foliar spraying with salicylic acid,so as to determine the effects of salicylic acid on resistance physiology of sweet glutinous corn seedlings under low temperature and salt stress.The results showed that the normal growth of sweet glutinous maize seedlings was significantly inhibited by 3℃low temperature and 100 mmol/L NaCl treatment.Spraying with different concentrations of salicylic acid can effectively alleviate the adverse effects of stress on the seedlings,with the best effect of 40 mg/L SA treatment.Spraying 40 mg/L salicylic acid caused the plant height,dry matter accumulation,relative water content,soluble sugar and soluble protein content of maize seedlings increased to varying degrees,and the MDA content was decreased.Both CAT and POD activities of maize seedlings with 40 mg/L SA treatment were all increased.Therefore,it is speculated that foliar spraying with salicylic acid may enhance the osmotic regulation ability of plants under stress conditions,enhance the activity of antioxidant enzymes,reduce membrane damage,and comprehensively improve the cold and salt resistance of sweet glutinous maize seedlings.

关 键 词：玉米 水杨酸 低温胁迫 盐胁迫 抗性生理 

分 类 号：S513[农业科学—作物学] Q945.78[生物学—植物学]