跨膜蛋白Klotho与纤连蛋白在瘢痕疙瘩表达水平及对术后复发的相关性分析  

Correlation Analysis of the Expression Levels of Transmembrane Protein Klotho and Fibronectin in Keloids and Their Relationship with Postoperative Recurrence

作　　者：孙艳萍[1] 刘鹏超 胡金宏 梁亚坤 韩福生[1] 唐海龙 缪国东 马志红[1] 郭有新[1] SUN Yanping;LIU Pengchao;HU Jinhong(Chengde Central Hospital,Hebei Chengde 067000,China)

机构地区：[1]河北省承德市中心医院,河北承德067000 [2]河北省承德市生态环境检验检测站,河北承德067000

出　　处：《河北医学》2024年第8期1331-1336,共6页Hebei Medicine

基　　金：河北省承德市科学技术研究与发展计划项目,(编号:202201A111)。

摘　　要：目的:探究Klotho蛋白结合纤连蛋白(FN)在瘢痕疙瘩术后复发的相关性。方法:选取2020年4月至2023年4月于我科就诊的耳部瘢痕疙瘩患者43例及普通求美者37例,实验对象分为研究组(瘢痕疙瘩组织和血清)及对照组(正常组织和血清)。采用免疫组织化学染色法及酶联免疫吸附法(ELISA)检测比较两组组织和血清标本中Klotho蛋白及上皮间质转化标志物纤连蛋白FN的表达。同时在术后1月、3月、6月、12月对瘢痕疙瘩患者进行随访、记录复发情况,分为复发组及未复发组。应用统计学方法分析Klotho的表达与瘢痕疙瘩纤维化标志物FN的表达及术后复发的相关性。结果:免疫组化结果:研究组Klotho阳性率较对照组低(P<0.05),为37.20%;研究组FN阳性率为72.10%,高于对照组阳性率(P<0.05);复发组Klotho阳性率为21.40%,低于未复发组(P<0.01);复发组FN阳性率为92.90%,高于未复发组(P<0.05)。Klotho和FN两种蛋白水平的相关性存在统计学差异(r=-0.307,P<0.05);ELISA检测血清结果:研究组Klotho表达含量为65.99(55.05,79.06)ng/mL,低于对照组(P<0.05);研究组FN表达含量为83.56(62.67,91.85)ng/mL,较高于对照组(P<0.01);经Logrank检验,在术后复发患者中Klotho阴性表达与FN蛋白阳性表达相关(P均<0.05)。结论:Klotho蛋白结合上皮间质转化标志物FN可作为预测瘢痕疙瘩术后复发的重要标志物。Objective:To investigate the correlation between Klotho protein binding to fibronectin Fn and postoperative recurrence in keloids.Methods:A total of 43 patients with ear keloids and 37 general cosmetic seekers who visited our department from April 2020 to April 2023 were selected.The subjects were divided into a study group(keloid tissues and serum)and a control group(normal tissues and serum).Immunohistochemistry and enzyme-linked immunosorbent assay(ELISA)were used to detect and compare the expression of Klotho protein and the epithelial-mesenchymal transition marker fibronectin FN in tissue and serum samples from both groups.Keloid patients were followed up at 1 month,3 months,6 months,and 12 months postoperatively to record recurrence,which divided them into recurrence and non-recurrence groups.Statistical methods were used to analyze the correlation between Klotho expression and fibronectin FN expression and postoperative recurrence in keloids.Results:Immunohistochemistry results showed that the positive rate of Klotho in the study group was lower than in the control group(P<0.05),at 37.20%.The positive rate of FN in the study group was 72.10%,higher than in the control group(P<0.05).The positive rate of Klotho in the recurrence group was 21.40%,lower than in the non-recurrence group(P<0.01),and the positive rate of FN in the recurrence group was 92.90%,higher than in the non-recurrence group(P<0.05).There was a statistically significant correlation between the levels of Klotho and FN proteins(r=-0.307,P<0.05).Elisa results showed that the expression level of Klotho in the study group was 65.99(55.05,79.06)ng/mL,lower than in the control group(P<0.05),and the expression level of FN in the study group was 83.56(62.67,91.85)ng/mL,higher than in the control group(P<0.01).Logrank tests indicated that negative Klotho expression and positive FN protein expression were associated with postoperative recurrence(both P<0.05).Conclusion:The combination of Klotho protein and the epithelial-mesenchymal transition marker

关 键 词：KLOTHO蛋白 上皮间质转化 纤连蛋白 瘢痕疙瘩 

分 类 号：R622[医药卫生—整形外科]