泽曲明山复方对大鼠非酒精性脂肪性肝炎模型影响  

Effect of Zequ Ming Shan Decoction(泽曲明山复方)on Mouse Model of Non-alcoholic Steatohepatitisy

作　　者：郑佳连[1] 张红 卢秉久[3] 张艳[3] ZHENG Jialian;ZHANG Hong;LU Bingjiu;ZHANG Yan(Liaoning University of Tradtional Chinese Medicine,Shenyang 110847,Liaoning,China;Anshan Mental Health Centre,Anshan 114001,Liaoning,China;Affiliated Hospital of Liaoning University of Tradtional Chinese Medicine,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]鞍山市精神卫生中心,辽宁鞍山114001 [3]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2024年第9期6-11,共6页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(82174241);辽宁省自然科学基金资助计划项目(2019-MS-231)。

摘　　要：目的泽曲明山复方是辽宁省名中医卢秉久教授治疗代谢相关性脂肪性肝病(metabolic associated fatty liver disease,MAFLD)的经验方。研究基于PI3K/AKT/mTOR通路探讨该经验方对大鼠非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,NASH)模型的影响。方法适应性饲养1周后,所有SD大鼠随机分为5组:对照组,模型组,中药低、高剂量组及水林佳组。对照组给予胆碱充足高脂饲料(choline-sufficient high fat diet,CSHF饲料)。其余4组大鼠给予胆碱缺乏高脂饲料(choline-deficiency high fat diet,CDHF饲料)8周建立NASH模型,建立模型同时给予对应药物干预。观察相应药物对模型大鼠血清生化学指标、肝脏病理学检查、PI3K/AKT/mTOR信号转导通路mRNA以及蛋白表达的影响。结果(1)体质量以及肝脏系数:5组大鼠体质量比较差异无统计学意义;与对照组比较,模型组大鼠肝脏系数显著升高;与模型组比较,各药物干预组大鼠肝脏系数不同程度下降,中药高剂量组疗效最为明显。(2)肝功能以及血脂:与对照组比较,模型组大鼠血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、甘油三酯(triglyceride,TG)、总胆固醇(cholesterol,CHOL)水平显著升高;与模型组比较,各药物干预组大鼠肝功能以及血脂指标水平不同程度降低,其中中药高剂量组疗效最为明显。(3)肝脏病理学检查:模型组大鼠肝脏HE染色可见胞质内大量脂肪空泡以及炎症细胞浸润;Sirius red染色可见中央静脉大量纤维组织沉积,证实造模成功;与模型组比较,各药物干预组大鼠肝脏HE以及Sirius red染色显示肝脏组织均有不同程度改善,中药高剂量组改善最为明显。(4)肝脏相关mRNA及蛋白表达:与对照组比较,模型组大鼠肝脏组织中PI3K、AKT以及mTOR mRNA表达明显升高;与模型组比较,各药物干预组mRNA表达有不同程度下调,其中中药高剂量组最为明显。与对照组�Objective Zequ Mingshan Decoction(泽曲明山复方)is an empirical formula for the treatment of metabolic associated fatty liver disease(MAFLD)by Professor LU Bingjiu,a renowned traditional Chinese medicine practitioner in Liaoning province.This study is based on the PI3K/AKT/mTOR pathway to explore the effect of this empirical formula on the rat model of non-alcoholic steatohepatitis(NASH).Methods After one week of adaptive feeding,all rats were randomly divided into five groups:control group,model group,low-dose Chinese medicine group,high-dose Chinese medicine group,and Shuilinjia group.To observe the effects of corresponding drugs on serum biochemistry,liver pathology,PI3K/AKT/mTOR signaling pathway mRNA and protein expression in model rats.Results(1)Body weight and liver index:There was no statistically significant difference in body weight among the 5 groups of rats.the liver index of the model group rats significantly increased;while the liver index of each drug intervention group rats decreased to varying degrees,the high-dose Chinese medicine group had the most significant therapeutic effect.(2)Liver function and blood lipids:Compared with the control group,the serum ALT,AST,TG,CHOL of the model group rats were significantly increased;Compared with the model group,the liver function and blood lipids of rats in each drug intervention group decreased to varying degrees,with the high-dose Chinese medicine group having the most significant therapeutic effect.(3)Liver pathological examination:HE staining showed a large number of fat vacuoles and inflammatory cell infiltration in the cytoplasm of the liver of the model group rats;Sirius red staining showed a large amount of fibrous tissue deposition in the central vein,confirming the validity of the model;Compared with the model group,each drug intervention group showed varying degrees of improvement in liver HE and Sirius red staining,with the high-dose Chinese medicine group showing the most significant improvement.(4)Liver related mRNA and protein express

关 键 词：泽曲明山复方 非酒精性脂肪性肝炎 PI3K/AKT/mTOR通路 

分 类 号：R256.4[医药卫生—中医内科学]