青海地区成年藏族活动性肺结核患者与汉族活动性肺结核患者肠道菌群差异研究  

Differences in gut microbiota between adult Tibetan active tuberculosis patients and Han active tuberculosis patients in Qinghai Province

作　　者：王玉清 姚杰 王玲 马晓磊 冶赓祉 程国栋 史发鹏 郑立恒[3] WANG Yuqing;YAO Jie;WANG Ling;MA Xiaolei;YE Gengzhi;CHENG Guodong;SHI Fapeng;ZHENG Li-heng(The Fourth People′s Hospital of Qinghai Province,Xining,Qinghai 810007,China;Medical College of Qinghai University,Xining,Qinghai 810001,China;Clinical Laboratory,Beijing Chest Hospital,Capital Medical University,Beijing 101149,China)

机构地区：[1]青海省第四人民医院,青海西宁810007 [2]青海大学医学院,青海西宁810001 [3]首都医科大学附属北京胸科医院检验科,北京101149

出　　处：《临床肺科杂志》2024年第9期1315-1320,共6页Journal of Clinical Pulmonary Medicine

基　　金：青海省第四人民医院课题(No.ynzz-2021-01)。

摘　　要：目的 分析青海地区成年藏族活动性肺结核患者与汉族活动性肺结核患者肠道菌群特点。方法 选取2021年12月至2022年6月在青海省第四人民医院诊断为活动性肺结核的患者12例,其中藏族8例,汉族4例。本研究采用16S rDNA测序技术,对两组研究对象的粪便样本进行16S rDNA测序,分析不同种群的肠道微生物群落结构及差异。使用线性判别分析(Linear Discriminant Analysis Effect Size, LefSe)探索特定菌群是否可以作为区分藏族与汉族肺结核患者的差异菌。结果 (1)α多样性分析结果表明,藏族与汉族肺结核患者的肠道菌群物种丰富度(Chao1指数,ACE指数)和多样性(Shannon指数,Simpson指数)差异无统计学意义(P>0.05);β多样性分析显示,藏族与汉族肺结核患者肠道菌群的多样性差异无统计学意义(P>0.05)。(2)未发现有差异菌门,但存在一定的属之间的差别。藏族与汉族肺结核患者在属级别中相比,粪芽孢菌属(1.031%vs 0.061%,P=0.003)、帕拉普氏菌属(0.175%vs 0.009%,P=0.011)、优杆菌属(0.248%vs 0.011%,P=0.004)、链型杆菌属(2.668%vs 0.007%,P=0.007)、霍尔德曼氏菌属(1.289%vs 0.011%,P=0.016)、芽殖菌属(0.511%vs 0.078%,P=0.035)、厌氧菌属(0.381%vs 0.008%,P=0.038)、多尔氏菌属(1.017%vs 0.305,P=0.046)和斯莱克氏菌属(0.100%vs 0,P=0.044)等菌属相对丰度的差异有统计学意义。(3)LefSe显示,肠道菌群在藏族肺结核患者组中富集的为优杆菌属、梭状芽孢杆菌属和厚壁菌门等,而肠道菌群在汉族肺结核患者组中富集的菌为双歧杆菌目、双歧杆菌属和双歧杆菌科等。结论 青海地区成年藏族与汉族肺结核患者的菌群多样性与丰富度可能无差异,但菌群组成及优势菌群可能存在差异性。Objective To study the characteristics of intestinal microbiota between the active Tibetan adults and Han active tuberculosis patients in Qinghai province.Methods From December 2021 to June 2022,12 cases of active pulmonary tuberculosis were performed in the Fourth People′s Hospital of Qinghai Province,including 8 Tibetan and 4 Han patients.16S rDNA gene sequencing techniques were used to detect the the structure and differences of gut microbiota communities among different populations of fecal samples from two groups.Linear discriminant analysis effect size(LEfSe)was selected to explore whether specific bacterial populations can serve as differential bacteria for distinguishing between Tibetan and Han tuberculosis patients.Results(1)The results ofαdiversity analysis indicated that there were no statistically significant differences in the Chao1 index(P=0.138),ACE index(P=0.139),Shannon index(P=0.103),and Simpson index(P=0.089)reflecting the species richness and diversity of gut microbiota between Tibetan and Han patients.βDiversity analysis showed that there was no statistically significant difference in the diversity of gut microbiota between Tibetan and pulmonary tuberculosis patients(P>0.05).(2)There was no differential phylum Intestinal flora,but there were certain differences among the genus.At the genus level,there was a statistically significant difference in relative abundance among Coprococcus(1.031%vs 0.061,P=0.003),Paraprevotella(0.175%vs 0.009%,P=0.011),Eubacterium(0.248%vs 0.011%,P=0.004),Catenobacterium(2.668%vs 0.007%,P=0.007),Holdemanella(1.289%vs 0.011,P=0.016),Gemmiger(0.511%vs 0.078%,P=0.035),Anaerobacteria(0.381%vs 0.008%,P=0.038),Dorea(1.017%vs 0.305,P=0.046)and Slackia(0.100%vs 0,P=0.044)between Tibetan and Han tuberculosis patients.(3)According to LefSe,the gut microbiota enriched in the Tibetan tuberculosis patient group are Eubacterium,Clostridia,and Firmicutes,etc.While the Han tuberculosis patient group were riched in Bifidobacteriaceae,Bifidobacterium,and Bifidobacteriaceae,etc.Co

关 键 词：16S rDNA基因测序 汉族 藏族 肠道菌群 肺结核 

分 类 号：R521[医药卫生—内科学]