谷子CPP基因家族鉴定及外源硒响应分析  

Identification of the CPP gene family and its response to exogenous selenium in foxtail millet

作　　者：杨馥熔 杜冰 郭浩杰 王成 张富厚[1] 孟超敏[1] YANG Furong;DU Bing;GUO Haojie;WANG Cheng;ZHANG Fuhou;MENG Chaomin(He nan Dryland Crop Germplasm Resources Utilization Engineering Research Center,College of Agriculture,He nan University of Science and Technology,Luoyang,He nan 471000,China)

机构地区：[1]河南科技大学农学院,河南省旱地作物种质资源利用工程研究中心,河南洛阳471000

出　　处：《西北植物学报》2024年第8期1250-1260,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：河南省产业科技特派员项目(2018HNCYTPY01);河南省旱地绿色智慧农业特色骨干学科群建设项目(17100001)。

摘　　要：【目的】探究谷子CPP基因家族成员特征及在外源硒处理下的响应模式,为谷子富硒高叶酸新品种选育提供遗传材料。【方法】借助生物信息学工具鉴定谷子CPP家族成员,用qRT-PCR技术对CPP基因在不同组织及外源硒处理下的表达水平进行检测。【结果】(1)谷子基因组中包含9个CPP基因,定位于6条染色体上,分别命名为SiCPP1-SiCPP9,所有成员均定位在细胞核,蛋白质二级结构中无规则卷曲占比最重。(2)谷子CPP蛋白可分为4个亚家族,相同亚家族之间保守基序和结构域的数目及分布相似。(3)启动子分析发现谷子CPP家族中存在大量光、生长发育、激素及胁迫响应元件。(4)谷子CPP家族成员在根、茎、叶和穗中差异表达,SiCPP5、SiCPP6、SiCPP7和SiCPP8对外源硒响应最强烈。【结论】谷子CPP家族成员具有组织表达特异性,且对外源硒存在不同程度响应。[Objective]The study aims to characterize CPP gene family in foxtail millet(Setaria italica L.)and analyze their response to exogenous selenium treatment,in order to provide materials for the breeding of new foxtail millet varieties with high selenium and folate.[Methods]This study used bioinformatics tools to identify the CPP gene family members in foxtail millet,and used qRT-PCR to detect their expression levels in different tissues under exogenous selenium treatment.[Results](1)9 CPP genes were identified in foxtail millet genome,which were distributed on 6 chromosomes,named SiCPP1-SiCPP9,respectively.These genes were located in the nucleus.The proportion of random coil in protein secondary structure was the largest.(2)The CPP protein in foxtail millet could be divided into four subfamilies,and the number and distribution of conserved motifs and domains were similar among the same subfamilies.(3)Cis-acting element analysis found that the SiCPP genes had a large number of light,growth and development-related,and hormone and stress response elements.(4)SiCPP genes were expressed differently in roots,stems,leaves,and ears,and SiCPP5,SiCPP6,SiCPP7 and SiCPP8 showed the strongest response to exogenous selenium.[Conclusion]SiCPP genes show tissue-specific expression and respond to exogenous selenium to varying degrees.

关 键 词：谷子 CPP基因家族 外源硒 生物信息学分析 表达模式 

分 类 号：Q789[生物学—分子生物学] S515[农业科学—作物学]