甘蓝型油菜种子硫代葡萄糖苷含量的QTL定位及候选基因分析  

QTL mapping and candidate gene identification of seed glucosinolate content in Brassica napus

作　　者：赵卫国 塔娜 王灏[2] ZHAO Weiguo;TA Na;WANG Hao(School of Modern Agriculture and Biotechnology,Ankang University,Ankang,Shaanxi 725000,China;The Rapeseed Hybrid Centerof Shaanxi Province,Yangling,Shaanxi 712100,China)

机构地区：[1]安康学院现代农业与生物科技学院,陕西安康725000 [2]陕西省杂交油菜研究中心,陕西杨凌712100

出　　处：《西北植物学报》2024年第8期1261-1272,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：陕西高校青年创新团队项目(2023AYTD01);陕西省大学生创新创业训练计划项目(S202311397010);陕西省重点研发计划项目(2020ZDLNY04-01);陕西省茶产业协同创新项目(23JY001,23JY003);安康学院高层次人才引进项目(2021AYQDZR12)。

摘　　要：【目的】解析甘蓝型油菜种子硫代葡萄糖苷性状的重要遗传位点及候选基因,为甘蓝型油菜硫苷基因克隆和品种品质改良奠定基础。【方法】以甘蓝型油菜KN DH群体种子为材料,对在4个种植环境的种子硫苷含量进行QTL定位和候选基因分析。【结果】(1)甘蓝型油菜种子硫苷含量变异系数较高且较为稳定,服从数量性状的遗传特点。(2)7个一致性QTL(cqGC.A9-5、cqGC.A9-7、cqGC.A9-9、cqGC.C2-9、cqGC.C2-10、cqGC.C9-5和cqGC.C9-6)为环境稳定表达QTL,包括3个主效QTL(cqGC.A9-5、cqGC.C2-10和cqGC.C9-5)。(3)在主效QTL cqGC.A9-5和cqGC.C9-5鉴定到3个候选基因(BnaA09g05480D、BnaC09g05620D和BnaC09g05810D),主要涉及硫苷生物合成途径中吲哚-3-乙醛肟、3-烷基-苹果酸的合成及硫苷的转运与分配。【结论】甘蓝型油菜种子硫苷含量为数量性状。3个甘蓝型油菜硫苷含量主效QTL被鉴定到,其置信区间候选基因的拟南芥同源基因参与硫苷合成途径中间产物合成及促进硫苷的转运与分配。[Objective]The study aims to discover the genetic loci and candidate genes of seed glucosinolate content in Brassica napus,and lay a foundation for the cloning of genes involved in glucosinolate metabolism and improving the breeding of B.napus.[Methods]Using KN DH populations from four different environments as materials,the QTL mapping of seed glucosinolate content and the candidate genes were analyzed.[Results](1)The variation coefficient of seed glucosinolate content in B.napus was high and stable,which followed the genetic characteristics of quantitative traits.(2)Seven consistent QTLs,including cqGC.A9-5,cqGC.A9-7,cqGC.A9-9,cqGC.C2-9,cqGC.C2-10,cqGC.C9-5 and cqGC.C9-6,were environmentally stable QTLs,of which cqGC.A9-5,cqGC.C2-10,and cqGC.C9-5 were major QTLs.(3)Three candidate genes,including BnaA09g05480D,BnaC09g05620D,and BnaC09g05810D,were identified in the interval of the major QTL cqGC.A9-5 and cqGC.C9-5.Based on their annotation,these candidate genes were involved in the biosynthetic pathway of glucosinolates(the biosynthesis of IAOx and 3-alkyl malic acid)and transport and distribution of glucosinolates.[Conclusion]Seed glucosinolate content in B.napus was quantitative trait.Three major QTLs for seed glucosinolate content were identified,which might be involved in the synthesis of intermediate products and the transport and distribution of glucosinolates.

关 键 词：甘蓝型油菜 种子硫苷含量 QTL定位 候选基因 

分 类 号：S565.4[农业科学—作物学]