山桐子雌、雄株叶片表皮微形态特征比较  

Comparison of the leaf epidermal micromorphology between female and male Idesia polycarpa Maxim

作　　者：尹丹丹 苏上 YIN Dandan;SU Shang(State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,China;Research Institute ofForestry,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院林木遗传育种全国重点实验室,北京100091 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《西北植物学报》2024年第8期1312-1320,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：院企合作专项(C286)。

摘　　要：【目的】探究山桐子叶片表皮微形态特征及其雌、雄株差异,为该种质资源的挖掘利用提供参考。【方法】利用扫描电镜对40份山桐子(雌、雄株各半)叶片进行微形态观察,测定12个气孔相关性状,并分析样品间的异同。【结果】(1)山桐子雌、雄株叶片的表皮毛、细胞形态、气孔特征及蜡质式样相似,其下表皮密布的类锥形纤维体型蜡质纹饰,鲜见于其他植物类群。(2)山桐子雌株叶片的气孔大小(长度、宽度、面积及周长)及密度相关参数均略大于雄株;雄株叶片的气孔开口相关参数(孔隙宽度、开口面积及气孔开度)均略高于雌株;但所有差异均未达到统计学显著水平(P>0.05)。(3)多数气孔性状彼此显著相关,而气孔密度与其他性状关联较弱,可能受到相对独立的遗传调控。【结论】研究明确了山桐子叶片表皮的微形态特征,其类锥形纤维体型蜡质式样可作为种质评鉴的关键依据,其气孔性状参数可为童期种苗的性别判定提供重要参考。[Objective]The study aims to explore the micromorphological characteristics of leaf epidermis of Idesia polycarpa Maxim.and investigate differences between the female and male plants in order to provide references for the mining and utilization of its germplasm resources.[Methods]The leaf epidermal micro-configurations of 40 plants of I.polycarpa(half female and half male)were characterized with scanning electron microscopy.12 stomatal traits were measured and the similarities and differences among the samples were analyzed.[Results](1)The leaf epidermal hair,cell morphology,stomatal characteristics,and wax pattern of both female and male I.polycarpa were similar.The dense,conical conicoid-like wax covered on the abaxial epidermis was rarely observed in other plants.(2)The leaf stomatal size(length,width,area,and perimeter)and density-related parameters of the female I.polycarpa were slightly higher than those of the male samples.On the contrary,leaf stomatal pore-related parameters(stomatal pore width,stomatal opening area,and stomatal opening level)were slightly higher in the male samples.But all differences were not statistically significant(P>0.05).(3)Most stomatal traits were significantly correlated with each other,while stomatal density was rarely related with others,which might be subject to relatively independent genetic regulation.[Conclusion]This study examined the characteristics of leaf epidermal micromorphology of I.polycarpa,and finded that the conical conicoid-like wax pattern could be used for germplasm evaluation,and the stomatal parameters were essential for gender determination of its young seedlings.

关 键 词：山桐子 表皮微形态 蜡质 气孔 

分 类 号：Q944.1[生物学—植物学]