基于抗坏血酸调控氧化铈纳米酶催化活性的抗坏血酸氧化酶检测研究  

Detection of Ascorbate Oxidase by Regulating the Catalytic Activity of Cerium Oxide Nano-Enzyme Based on Ascorbic Acid

作　　者：潘蕊 黄伟华 李佳娜 王丽丽 朱希[3] 许惠凤 PAN Rui;HUANG Weihua;LI Jiana;WANG Lili;ZHU Xi;XU Huifeng(Fujian Key Laboratory of Integrative Medicine on Geriatrics,Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122;College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,350122;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002)

机构地区：[1]福建省中西医结合老年性疾病重点实验室,福建中医药大学中西医结合研究院,福建福州350002 [2]福建中医药大学药学院,福建福州350002 [3]福建农林大学生命科学学院,福建福州350002

出　　处：《分析科学学报》2024年第4期389-394,共6页Journal of Analytical Science

基　　金：福建省自然科学基金(No.2021J01091,No.2023J01152);福建中医药大学校管课题重点项目(X2022007)。

摘　　要：纳米酶是一类既有纳米材料的独特性能又有催化功能的模拟酶,它既有天然酶的高催化活性,又有模拟酶稳定经济的特点,在生物传感检测领域的应用十分广泛。本研究采用水热法合成具有辣根过氧化物酶样活性的氧化铈纳米酶。该纳米氧化铈同时具有+4价和+3价两种形态,其中Ce^(4+)能与过氧化氢反应,使3,3’,5,5’-四甲基联苯胺(TMB)氧化,溶液显蓝色。由于抗坏血酸(AA)能还原Ce^(4+),抑制其催化活性以及TMB的氧化,从而使溶液的颜色较浅。而抗坏血酸氧化酶可催化氧化AA,阻碍了其对Ce^(4+)的还原,使纳米氧化铈的催化性能恢复。利用这种方法,实现对抗坏血酸氧化酶的定量检测,在12 U/L~400 U/L范围内呈良好的线性关系(R^(2)=0.9704),检测限为3.2 U/L。该研究为基于氧化铈纳米酶的比色方法在抗坏血酸氧化酶活性检测及相关应用提供基础。Nanozyme is a kind of simulated enzyme that has both the unique properties of nanomaterials and enzyme like catalytic activity.It owns the high catalytic activity of natural enzymes and the characteristics of economy and stability of simulated enzymes.Nanozymes have been widely applied in the field of bioanalysis.Herein,cerium oxide nanoparticles(CeO_(2) NPs)with horseradish peroxidase activity were synthesized by simple hydrothermal method.The nano-sized CeO_(2) NPs have both+4 and+3 valence forms,in which+4 valence can react with hydrogen peroxide to oxidize 3,3,5,5’-tetramylbenzidine(TMB),making the solution turning blue.Ascorbic acid(AA)can reduce Ce^(4+)and inhibit its catalytic activity on H 2 O_(2),further suppress the oxidation of TMB,resulting in a lighter color of the solution.Ascorbic acid oxidase(AAO),which catalyzes the oxidation of AA,obstructs the reduction of Ce^(4+)and restores the catalytic activity of CeO_(2) NPs.Using this method,the quantitative detection of AAO was achieved in the range of 12 to 400 mU/mL(R^(2)=0.9704),and the detection limit was 3.2 mU/mL.This study provides a basis for the colorimetric method based on CeO_(2) NPs to detect the activity of AAO and its related applications.

关 键 词：氧化铈 抗坏血酸氧化酶 纳米酶 比色分析 

分 类 号：O657.3[理学—分析化学]