荧光桃红B稳定剂制备银纳米簇及用于盐酸小檗碱的检测  

Silver Nanoclusters Using Phloxine B as Stabilizer and the Application in the Detection of Berberine

作　　者：伍瑶 凌云芝 郑若男 龙云飞[1] WU Yao;LING Yunzhi;ZHENG Ruonan;LONG Yunfei(College of Chemistry and Chemical Engineering,Hunan University of Science and Technology,Xiangtan 411100)

机构地区：[1]湖南科技大学化学化工学院,湖南湘潭411100

出　　处：《分析科学学报》2024年第4期447-453,共7页Journal of Analytical Science

基　　金：国家自然科学基金(No.21275047)。

摘　　要：本文以荧光桃红B为稳定剂,硝酸银为前驱体,硼氢化钠为还原剂,混合反应4 h制备得到具有荧光特性的银纳米簇(AgNCs)。实验显示,所合成的AgNCs的最佳激发波长为339 nm,最佳发射波长为444 nm。研究发现盐酸小檗碱(BH)能使合成的AgNCs的荧光显著猝灭,当BH浓度在1.25~12.50μmol/L范围内时,AgNCs的荧光强度的猝灭值(ΔI F)与BH浓度之间呈良好的线性关系,相关系数r为0.9958。基于此建立了一种测定BH的荧光分析方法,该方法的检出限为0.41μmol/L。同时,以紫外灯对体系进行照射时,pH浓度在3.75~12.50μmol/L范围时,溶液的颜色与pH浓度之间呈现梯度变化,因此可以建立一种更为简单的可视化检测BH的半定量检测方法。A new silver nanocluster(AgNCs)was synthesized using phloxine B as a stabilizer,silver nitrate as the raw material,and sodium borohydride as a reducing agent for more than 4 hours.The AgNCs have the optimal excitation wavelength of 339 nm and the optimal emission wavelength of 444 nm,which exhibit blue fluorescence under ultraviolet lamp irradiation.Research has found that Berberine(BH)can significantly quench the fluorescence of AgNCs.At the same time,the change values of the AgNCs fluorescence intensity(ΔI F)showed a good linear relationship with the BH concentrations in the ranging from 1.25 to 12.50μmol/L with the detection limit of 0.41μmol/L.Thus,a simple and rapid method for detecting BH has been established.Further research has found that there is a gradient change between the color and concentration of the mixing solution in the range from 1.25 to 12.50μmol/L when the mixing solution is illuminated with a UV lamp,so a simpler visual semi-quantitative detection method for BH can be established.

关 键 词：荧光桃红B 银纳米簇 盐酸小檗碱 

分 类 号：O657.39[理学—分析化学]