四川不同寄主禾谷镰孢菌复合种的群体遗传多样性及相互侵染关系  

Population Genetic Diversity and Infection Relationship of Fusarium graminearum Species Complex from Different Hosts in Sichuan

作　　者：李晓迪 陈思怡 陈国亮 刘林文 陈华保[1] 杨春平[1] 常小丽[1,2] LI Xiaodi;CHEN Siyi;CHEN Guoliang;LIU Linwen;CHEN Huabao;YANG Chunping;CHANG Xiaoli(College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China;Sichuan Engineering Research Center for Crop Strip Intercropping System,Chengdu 611130,China)

机构地区：[1]四川农业大学农学院,成都611130 [2]四川省玉米大豆带状复合种植工程技术中心,成都611130

出　　处：《四川农业大学学报》2024年第4期752-761,共10页Journal of Sichuan Agricultural University

基　　金：四川省豆类杂粮创新团队(SCCXTD-2024-20)。

摘　　要：【目的】探明四川地区小麦、玉米和大豆3种寄主禾谷镰孢菌复合种(Fusarium graminearum species complex,FGSC)的群体遗传多样性及相互侵染关系。【方法】采用多重PCR和PCR-VNTR分子标记技术分析不同寄主来源菌株的毒素类型和群体遗传多样性,并通过高粱粒接种法检测菌株在不同寄主间的相互侵染作用。【结果】来源于3种寄主的禾谷镰孢菌复合种菌株被鉴定为禾谷镰孢菌(F. graminearum)和亚洲镰孢菌(F. asiaticum),其中禾谷镰孢菌的毒素化学型为3-乙酰基脱氧雪腐镰刀菌烯醇(3-ADON)和15-乙酰脱氧雪腐镰刀菌烯醇(15-ADON),而亚洲镰孢菌的毒素化学型为雪腐镰刀菌烯醇(NIV);各寄主菌株的群体遗传多样性存在差异,以小麦菌株群体遗传多样性最高,且与大豆菌株遗传距离较远;同时,各寄主菌株间能够相互侵染,但致病力存在差异。【结论】来源于四川小麦、玉米和大豆3种寄主的禾谷镰孢菌复合种遗传多样性存在差异,但可相互侵染致病,具有加重其所致病害在该地区发生的风险。【Objective】This study aims to investigate the population genetic diversity and the mutual infection relationship of Fusarium graminearum species complex(FGSC)from three hosts including wheat,maize and soybean in Sichuan.【Method】The mycotoxin types and population genetic diversity were analyzed by multiple PCR and PCR-VNTR,while the pathogenicity of different strains on three hosts were tested using sorghum grain inoculation method.【Result】FGSC strains from three hosts were identi‐fied as F.graminearum and F.asiaticum.F.graminearum produces 3-acetyldeoxynivalenol(3-ADON)and 15-acetyldeoxynivalenol(15-ADON),while F.asiaticum produces only nivalenol(NIV).Population genetic analysis by PCR-VNTR showed that strains from wheat had the highest genetic diversity among three hosts,strains between wheat and soybean had the biggest genetic distance.Meanwhile,all FGSC strain can infect three hosts but vary in aggressiveness.【Conclusion】This study reveals that the genetic diversity of FGSC among wheat,maize and soybean has significant difference but they can cause diseases among three hosts in Sichuan,which largely increased the risk of disease occurrence caused by FGSC in this region.

关 键 词：禾谷镰孢菌复合种 毒素基因型 群体遗传多样性 致病性 

分 类 号：S432.4[农业科学—植物病理学]