4种作物果糖激酶基因的鉴定与比较分析  

Identification and Comparative Analysis of Fructokinase Genes in Four Crops

作　　者：刘胤如 鲁佳林 唐科 李莹莹 周润 刘汉梅[1] LIU Yinru;LU Jialin;TANG Ke;LI Yingying;ZHOU Run;LIU Hanmei(College of Life Science,Sichuan Agricultural University,Yaan 625014,Sichuan,China)

机构地区：[1]四川农业大学生命科学学院,四川雅安625014

出　　处：《四川农业大学学报》2024年第4期780-791,共12页Journal of Sichuan Agricultural University

基　　金：国家自然科学青年基金(31601317)。

摘　　要：【目的】基于全基因组序列,鉴定与分析水稻、玉米、高粱和谷子4种作物的果糖激酶(fructokinase, FRK)家族基因,明确其复制起源方式、系统发生关系、序列及表达分化特征等,为深入开展FRK基因功能研究、改良作物产量和品质等提供数据。【方法】采用基因组搜索、基因组内及组间比较等多种方法,分析4种作物FRK家族成员的基因结构、保守基序、系统进化、复制扩张和基因表达分化等。【结果】鉴定到水稻13个、玉米16个、高粱14个、谷子16个FRK基因家族成员,其中水稻11个、玉米7个、高粱12个、谷子13个基因为离散复制基因,水稻、高粱和谷子各有1对ρWGD基因,玉米有8个WGD基因(1个ρWGD基因,7个mWGD基因)。该家族成员亚家族间基因结构差异大,但在亚家族内具有保守性。基因表达模式分析发现,直系同源基因间的表达模式相似,ρWGD基因对发生了明显的表达分化,而玉米物种特异的mWGD基因对的表达差异较小。【结论】探明了4种作物基因组包含的FRK家族基因,揭示了其复制起源、基因结构、序列及表达模式的演化特征。【Objective】Based on the whole genome data,the gene identification and analysis of fructoki-nase(FRK)family in four crops(rice,maize,sorghum and foxtail millet)were conducted to clarify the modes of origin duplication,phylogenetic relationship and the characteristics of sequence and expression divergence.The findings of this study will provide data for the functional research on fructokinase and for enhancing crop yield and quality.【Method】The gene structure,conserved motifs,phylogeny,duplica-tion expansion,and gene expression divergence of fructokinase family members in four crops were ana-lyzed using whole genome search,and intra-and inter-genomic comparisons.【Result】A total of 13,16,14 and 16 FRK genes were identified in rice,maize,sorghum and foxmail millet,respectively.Among these,11 in rice,7 in maize,12 in sorghum,and 13 in foxmail millet were generated by dis-persed duplication,while one pair of genes underwent duplication throughρwhole genome duplication(ρWGD)in rice,sorghum and foxmail millet.Eight WGD genes(oneρWGD and seven mWGD genes)were identified in maize.The gene structures of fructokinase family varied widely between inter-subfamilies,yet remained conserved within intra-subfamilies.The gene expression analysis revealed that orthologs displayed similar expression patterns,while paralogs,especially the gene pairs generated byρWGD,exhibited divergent expression pattern.Minor differences were observed among paralogs dupli-cated by mWGD.【Conclusion】This study delved into the exploration of genes encoding fructokinase in the genomes of four crops,unveiling insights into duplication modes,gene structures,phylogenetic characteristics of sequences,and expression patterns.

关 键 词：禾本科作物 果糖激酶基因 基因复制 序列及表达分化 

分 类 号：S511[农业科学—作物学] S513S514S515