铝胁迫下千年桐内生真菌对根际土壤不同铝形态的影响  

Effects of Endophytic Fungi in Aleurites montana on Different Aluminum Forms in Rhizosphere Soil under Aluminium Stress

作　　者：蔡鑫伟 张瑶锐 林晗[1,2] 陈灿 范海兰[1,2] 谢安强 张斌[2,3] CAI Xinwei;ZHANG Yaorui;LIN Han;CHEN Can;FAN Haian;XIE Anqiang;ZHANG Bin(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Lab for Forest Ecosystem Processes and Management in Fujian Province,Fuzhou 350002,China;Forestry Bureau of Jianyang District,Nanping 354200,Fujian,China)

机构地区：[1]福建农林大学林学院,福州350002 [2]森林生态系统过程与经营福建省高校重点实验室,福州350002 [3]南平市建阳区林业局,福建南平354200

出　　处：《四川农业大学学报》2024年第4期828-835,共8页Journal of Sichuan Agricultural University

基　　金：福建省科技厅计划项目(KTP21116A);福建省林业厅种业创新项目(KLb21009B);福建省水土保持试验站项目(KH190193A、KH220045A);福建农林大学林学高峰学科建设项目(72202200205);福州市自然科学基金项目(榕科[2021]266号)。

摘　　要：【目的】了解不同浓度铝胁迫下内生真菌对根际土壤不同铝形态的影响。【方法】将5种分离自千年桐的优势内生真菌菌株配置为菌液,分别施入不同浓度铝胁迫的千年桐盆栽苗,外源铝浓度设置为0 mmol/L (A0)、0.555 mmol/L (A1)、0.745 6 mmol/L (A2)、1.111 mmol/L (A3)和1.852 mmol/L (A4)。通过测定不同菌液处理的土壤活性铝含量以及千年桐根系活力,探讨内生真菌对根际土壤活性铝含量及比例变化的影响。【结果】在非铝胁迫(A0)的条件下,不同内生真菌对千年桐根际土壤活性铝含量均有不同程度的升高。施加链格孢属菌液处理后,根际土壤交换性铝含量在A2铝浓度时较未施菌处理降低了20.58%,单聚体羟基铝含量在各铝浓度下均低于未施菌处理,降幅分别为7.19%(A1)、19.56%(A2)、6.27%(A3)和4.01%(A4)。盾壳霉属和嗜热真菌属菌液处理后,千年桐根际土壤活性铝含量均高于未施菌处理。不同菌液处理对千年桐根系活力的作用各异,其中盾壳霉属处理后的根系活力较未施菌提升了11.27%,而拟盘多毛孢属处理后根系活力与未施菌处理无显著差异。【结论】接种内生真菌能改变植株根际土壤活性铝含量和比例。链格孢属能有效降低根际土壤单聚体羟基铝含量,而盾壳霉属和嗜热真菌属提升了根际土壤活性铝含量。内生真菌的作用效果受到铝浓度、内生真菌基因型和宿主种类的影响。研究结果拓展了内生真菌对于宿主根际土壤影响的探索,可为林木-微生物互作的深入研究提供参考。【Objective】This study aims to investigate the effects of endophytic fungi on different forms of aluminum in rhizosphere soil under different aluminium stress levels.【Method】Five dominant endo-phytic fungal strains isolated from Aleurites montana were cultured as fungal solutions and applied to pot-ted A.montana seedlings under different aluminum levels,including 0 mmol/L(A0),0.555 mmol/L(A1),0.7456 mmol/L(A2),1.111 mmol/L(A3)and 1.852 mmol/L(A4).The effects of endophytic fungi on the contents and proportions of different forms of active aluminum in rhizosphere soil were inves-tigated by measuring the contents of active aluminum in soil and the root activity of A.montana treated with different fungal solutions.【Result】Under non-aluminum stress(A0)condition,various endo-phytic fungi caused different levels of increase in the active aluminum contents in the rhizosphere soil of A.montana.The inoculation with Alternaria sp.led to a 20.58%reduction in exchangeable aluminum content in rhizosphere soil under A2 aluminum level compared to the non-inoculated treatments.Mean-while,the content of monomeric hydroxyl aluminum in Alternaria sp.-inoculated treatments was lower than that of the non-inoculated treatments across all aluminum levels,with reductions of 7.19%,19.56%,6.27%and 4.01%for A1,A2,A3 and A4,respectively.For Coniothyrium sp.and Thermomy-ces sp.treatments,the active aluminum contents in the rhizosphere soil were higher compared to the non-inoculated treatments.Different fungal inoculation treatments had varying effects on the root activity of A.montana,among which the root activity inoculated with Coniothyrium sp.was 11.27%higher than that of the non-inoculated treatments,while the root activity inoculated with Pestalotiopsis sp.showed no signifi-cant difference compared with that of non-inoculated treatments.【Conclusion】These findings show that inoculation with endophytic fungi could change the content and proportion of active aluminum in rhizo-sphere soil.Alternaria sp.could effectively reduce

关 键 词：内生真菌 千年桐 铝胁迫 活性铝 

分 类 号：S794.3[农业科学—林木遗传育种]