基于GBS简化基因组技术的蕲艾遗传多样性分析  

Genotyping-by-sequencing Reveals Genetic Diversity of Artemisia argyi Germplasm Resources

作　　者：陈昌婕 肖闯 马钰洋 苗玉焕 刘大会 CHEN Changjie;XIAO Chuang;MA Yuyang;MIAO Yuhuan;LIU Dahui(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Hubei Shizhen Laboratory,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,武汉430065 [2]湖北时珍实验室,武汉430065

出　　处：《中国实验方剂学杂志》2024年第18期171-177,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局青年岐黄学者支持项目;国家自然科学基金项目(32270391);湖北省中医药管理局2023-2024年度中医药科研项目(ZY2023Z023);湖北省自然科学基金项目(2023AFA032);现代农业产业技术体系建设专项(CARS-21);中央本级重大增减支项目(2060302)。

摘　　要：目的:探究45份艾种质资源的遗传关系,揭示其遗传变异特点。方法:基于基因分型(GBS)的简化基因组技术对45份资源进行了单核苷酸多态性(SNP)位点挖掘,并利用SNP标记进行了主成分分析、系统发育分析、群体遗传结构分析和遗传变异分析。结果:共获得111.91 Gb数据,测序结果的Q20和Q30分别为96.39%和90.33%,平均GC含量为39.37%,clean reads与参考基因组的比对率为70.24%~98.97%,共获得22 399个Indel和170 539个SNP位点,其中第10对染色体上的变异位点最多;主成分分析、聚类分析和遗传多样性分析表明,45份资源可以被分为3个类群,类群Ⅰ包含的3份资源均来源于蕲春县,第Ⅱ类群资源均为野生种,类群Ⅲ包含31份种质,来源最为复杂;同时,这45份种质可分为3亚类,包含来源于3个祖先的遗传信息,说明不同种源的艾遗传组成比较复杂,尤其是来源于湖北省蕲春县的种质。结论:该研究可为蕲艾的新品种选育、特异性SNP标记的开发、艾的亲缘关系研究提供理论支撑。Objective To explore the genetic relationship and reveal the genetic variations of 45 germplasm accessions of Artemisia argyi.Method Genotyping-by-sequencing(GBS)was employed to mine single nucleotide-polymorphisms(SNPs)from the 45 germplasm accessions.Principal component analysis,phylogenetic analysis,population genetic structure analysis,and genetic variation analysis were conducted based on the SNPs.Result A total of 111.91 Gb of data were obtained,with the Q20,Q30,and average GC content of 96.39%,90.33%,and 39.37%,respectively.The comparison rate between clean reads and the reference genome was 70.24%-98.97%.A total of 22399 Indels and 170539 SNPs were obtained,and the 10th pair of chromosomes had the most variation sites.The results of principal component analysis,cluster analysis,and genetic diversity analysis classified the 45 germplasm accessions into three groups.GroupⅠcontained three germplasm accessions from Qichun County.The germplasm accessions in groupⅡwere all wild.GroupⅢcontained 31 germplasm accessions,with the most complex sources.Moreover,the 45 germplasm accessions can be classified into 3 subtypes,containing the genetic information from three ancestors.The results indicated rich genetic diversity of A.argyi from different sources,especially the germplasm accessions from Qichun County,Hubei province.Conclusion This study provides theoretical support for breeding new varieties,developing specific SNP markers,and revealing the genetic relationship of A.argyi.

关 键 词：蕲艾 基因分型(GBS) 单核苷酸多态性(SNP)标记 遗传多样性分析 聚类分析 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学] R289R287R22R2-031R33R24