气相色谱法测定盐酸氯丙嗪注射液中5种抗氧化剂  

Determination of 5 antioxidants in chlorpromazine hydrochloride injection by gas chromatography

作　　者：朱玉婷 李灿 武蕊 彭国泰 唐文杰 邹子爵 陈同强 ZHU Yuting;LI Can;WU Rui;PENG Guotai;TANG Wenjie;ZOU Zijue;CHEN Tongqiang(3201 Hospital of Xi'an Jiaotong University Health Science Center,Hanzhong 723000,China;Hunan Province Produce Commodity Quality Testing&Research Institute,Changsha410007,China)

机构地区：[1]西安交通大学医学部附属三二〇一医院,陕西汉中723000 [2]湖南省产商品质量检验研究院,长沙410007

出　　处：《化学分析计量》2024年第8期7-11,共5页Chemical Analysis And Meterage

基　　金：湖南省自然科学基金项目(2022JJ90024,2022JJ90026)。

摘　　要：建立气相色谱法测定盐酸氯丙嗪注射液中5种抗氧化剂。采用乙酸乙酯作为提取溶剂,HP-1701毛细管色谱柱分离,分流比为10∶1,用氢火焰离子化检测器(FID)检测,色谱峰面积外标法定量。5种抗氧化剂能实现完全分离,其质量分数在0~100μg/mL范围内与色谱峰面积有良好的线性关系,相关系数均大于0.999,检出限为2.0~4.0mg/kg,定量限为5.0~9.0 mg/kg。样品加标回收率为89.36%~95.92%,测定结果的相对标准偏差均小于8%(n=6)。该方法可满足盐酸氯丙嗪注射液中5种抗氧化剂含量的检测。A gas chromatography method was established to determine five antioxidants in chlorpromazine hydrochloride injection.Ethyl acetate was used as the extraction solvent,separated by HP-1701 capillary chromatography column with a split ratio of 10∶1,detected by hydrogen flame ionization detector(FID),and quantified by chromatographic peak area external standard method.Five antioxidants could achieve complete separation,and their mass fractions had good linear relationships with chromatographic peak areas in the range of 0-100μg/mL,and correlation coefficients were more than 0.999.The detection limits were 2.0-4.0 mg/kg,and the quantification limits were 5.0-9.0 mg/kg.The recoveries of sample spiking were 89.36%-95.92%,the relative standard deviations of the measurement results were less than 8%(n=6).This method can meet the detection of 5 antioxidant contents in chlorpromazine hydrochloride injection.

关 键 词：气相色谱法 盐酸氯丙嗪注射液 抗氧化剂 

分 类 号：O657.7[理学—分析化学]