超高效液相色谱-三重四级杆线性离子阱串联质谱法测定化妆品中α-三联噻吩  

Determination ofα-terthiophene in cosmetics by UPLC-QTRAP-MS/MS

作　　者：马榕 平惠娟 胡伟杰 邵阳 马春艳 MA Rong;PING Huijuan;HU Weijie;SHAO Yang;MA Chunyan(Hebei Institute for Drug and Medical Device Control,Shijiazhuang 050227,China)

机构地区：[1]河北省药品医疗器械检验研究院,石家庄050227

出　　处：《化学分析计量》2024年第8期38-43,共6页Chemical Analysis And Meterage

基　　金：河北省中医药管理局科研项目(2024346)。

摘　　要：建立了超高效液相色谱-三重四级杆线性离子阱串联质谱测定化妆品中α-三联噻吩的检测方法。膏霜、乳液、水剂基质的化妆品样品经甲醇超声提取20 min,采用Agilent Infinitylab Poroshell 120 SB-C_(18)色谱柱,以0.1%(质量分数)甲酸水溶液-甲醇为流动相梯度分离,流量为0.3 mL/min,柱温为40℃。质谱配备电喷雾离子源,采用正离子多反应监测模式进行测定。在三种基质中,α-三联噻吩的质量浓度在0.002~0.2μg/mL范围内与色谱峰面积呈良好线性关系,相关系数大于0.999,检出限为0.008 mg/kg。低、中、高三个浓度水平的空白基质加标回收率为88.4%~100.4%,测定结果的相对标准偏差为1.8%~3.8%(n=6)。该方法为化妆品中α-三联噻吩的定性及痕量分析提供了一种快捷可靠的检测手段。An ultra-high performance liquid chromatography-triple quadrupole linear ion-trap mass spectrometry(UPLC-QTRAP-MS/MS)was developed for determination ofα-terthiophene in cosmetics.Cosmetics samples(cream,lotion and toner)were extracted with methanol for 20 min,separated with an Agilent Infinitylab Poroshell 120 SB-C_(18) column by gradient elution,eluted with 0.1%(mass fraction)formic acid aqueous solution-methanol as mobile phase at 40℃and a flow rate of 0.3 mL/min,then determined by MS equipped with an electrospray ion source in positive modes under the MRM mode.For all the 3 matrix samples,the masse concentration ofα-terthiophene had a good linearity with the concentration range of 0.002-0.2μg/mL,the correlation coefficients were more than 0.999,and the detection limits were 0.008 mg/kg.The average recoveries were 88.4%-100.4%,the relative standard deviations of the measurement results were 1.8%-3.8%(n=6).The method can be used as a efficient and reliable method for the qualitative and quantitative analysis and even trace analysis ofα-terthiophene in cosmetics.

关 键 词：三联噻吩 超高效液相色谱-三重四级杆线性离子阱串联质谱法 痕量分析 化妆品 

分 类 号：O657[理学—分析化学]