基于UPLC-Q-TOF-MS技术和网络药理学人参黄精杏麦饮化学成分群的抗疲劳作用研究  

Study on the anti-fatigue effect of the chemical constituents of Ginseng Huangjing Xing Mai Yin based on UPLC-Q-TOF-MS technology and network pharmacology

作　　者：刘秋容 许先梅 许震 刘廷云 钱志刚 谷丽维[1] 沈硕[1] 杜茂波[1] LIU Qiurong;WU Xianmei;XU Zhen;LIU Tingyun;QIAN Zhigang;GU Liwei;SHEN Shuo;DU Maobo(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;Wuhu Nuokang Biotechnology Co.,Ltd.,Wuhu 241000,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]沈阳药科大学中药学院,辽宁沈阳110016 [3]芜湖市诺康生物科技有限公司,安徽芜湖241000

出　　处：《沈阳药科大学学报》2024年第8期1097-1108,共12页Journal of Shenyang Pharmaceutical University

摘　　要：目的采用超高液相色谱-四极杆飞行时间串联质谱(UPLC-Q-TOF-MS)技术与网络药理学探讨人参黄精杏麦饮抗疲劳的药效物质及作用机制。方法通过UPLC-Q-TOF-MS技术鉴定人参黄精杏麦饮化学成分,并利用TCMSP、Swiss Target Prediction数据库预测化学成分相关靶点;利用Disgenet、Genecards数据库检索疲劳靶点,利用韦恩图绘制平台获取共有靶点,并将信息导入Cytoscope3.7.1软件和STRING在线分析平台,进行网络拓扑学分析,构建药物-活性成分-关键靶点网络。最后,通过DAVID数据库进行基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and gnomes,KEGG)通路富集分析。结果通过UPLC-Q-TOF-MS分析鉴定出35个药物活性成分,主要为黄酮类;对药物和疾病靶标集进行拓扑分析,获得14个关键成分和39个核心靶点;通过KEGG富集到癌症、流体剪切应力与动脉粥样硬化通路、PI3K-Akt、脂质与动脉粥样硬化和糖尿病并发症中的AGE-RAGE等信号通路。结论人参黄精杏麦饮的活性成分通过作用于丝氨酸/苏氨酸蛋白激酶1、磷酸甘油醛脱氢酶、白细胞介素-6、肿瘤坏死因子等靶标改善机体氧化应激反应、减轻免疫介导的炎症和感染以及调控细胞增殖、分化、凋亡等多种细胞功能发挥抗疲劳作用,初步阐明人参黄精杏麦饮抗疲劳的作用,为后续深入研究提供参考。Objective To explore the effective.components and mechanism of Ginseng Huangjing Xing Mai Yin in the treatment of fatigue based on UPLC-Q-TOF-MS technology and network pharmacology.Methods The chemical.components of Ginseng Huangjing Xing Mai Yin were qualitatively analyzed by UPLC-Q-TOF-MS technology,and the main.component targets were obtained by TCMSP database and Swiss Target Prediction;The targets of fatigue were searched using Disgenet and Genecards databases,the Venn diagram drawing platform was used to obtain.common targets was imported into Cytoscope 3.7.1 software and STRING online analysis platform,to conduct network topology analysis and construct drug-active ingredient-core target Dot network map.Finally,GO and KEGG pathway enrichment analysis was performed through the DAVID database.Results A total of 35 main.components of Ginseng Huangjing Xing Mai Yin were identified by UPLC-Q-TOF-MS,mainly flavonoids;A total of 14 key active ingredients and 39 core targets were obtained by a topology analysis;signaling pathways of cancer,fluid shear stress and atherosclerosis pathways,PI3K-Akt,lipids and atherosclerosis,AGE-RAGE and other signal pathways in diabetes.complications were obtained through the analysis of KEGG enrichment.Conclusions The active.components of Ginseng Huangjing Xing Mai Yin can improve the body′s oxidative stress response,alleviate immune mediated inflammation and infection,and regulate various cellular functions such as cell proliferation,differentiation,and apoptosis by acting on targets such as serine/threonine protein kinase 1,phosphoglyceraldehyde dehydrogenase,interleukin-6,and tumor necrosis factor,thus exerting anti fatigue effects.Thus,the anti-fatigue effect of Ginseng Huang Jing Xing Mai Yin is preliminary elucidated,which provides the reference for further in-depth research.

关 键 词：人参黄精杏麦饮 疲劳 UPLC-Q-TOF-MS技术 网络药理学 作用机制 

分 类 号：R28[医药卫生—中药学]