副溶血弧菌OpaR转录调控元研究  

Analysis of OpaR Transcription Regulon in Vibrio parahaemolyticus

作　　者：仇越 薛星帆 张苗苗 陆仁飞[3] 张义全 周敏[4] QIU Yue;XUE Xingfan;ZHANG Miaomiao;LU Renfei;ZHANG Yiquan;ZHOU Min(Department of Clinical Laboratory,The Affiliated Changzhou Hospital NO.2 People’s Hospital of Nanjing Medical University,Changzhou 213000,China;Department of Clinical Laboratory,The Affiliated Taizhou People’s Hospital of Nanjing Medical University,Taizhou 225399,China;Department of Clinical Laboratory,Nantong Third People’s Hospital,Affiliated Nantong Hospital 3rd of Nantong University,Nantong 226006,China;Department of Microbiology Laboratory,Nantong Center for Disease Control and Prevention,Nantong 226007,China)

机构地区：[1]南京医科大学附属常州第二人民医院检验科,江苏常州213000 [2]南京医科大学附属泰州人民医院检验科,江苏泰州225399 [3]南通市第三人民医院(南通大学附属南通第三医院)检验科,江苏南通226006 [4]南通市疾病预防控制中心微生物科,江苏南通226007

出　　处：《工业微生物》2024年第4期156-160,共5页Industrial Microbiology

基　　金：南通市卫生健康委员会科研课题(编号:QN2022044)。

摘　　要：文章旨在探究OpaR在生物膜形成条件下的转录调控机制。通过对野生型和opaR基因突变株的转录组数据进行比较,发现OpaR共调控了2243个基因的转录,其中1164个下调,1079个上调。功能富集分析结果显示,这些基因涉及分子功能、细胞组分、生物学过程等方面。代谢途径分析结果表明,差异表达基因主要涉及细胞代谢通路和环境信息处理。此外,从数据中还筛选出了多个与生物膜形成相关的基因。这些发现为理解OpaR调控生物膜形成的分子机制提供了重要信息和参考。This article aims to investigate the transcriptional regulation mechanism of OpaR regulon under the biofilm formation condition.By comparing the transcriptome data of wild-type and opaR gene mutant strains,it was found that OpaR regulates the transcription of 2243 genes,of which 1164 were down-regulated and 1079 were up-regulated.The functional enrichment analysis displayed that these genes involved molecular function,cellular components,biological processes,and other aspects.The metabolic pathway analysis revealed that differentially expressed genes mainly involved cellular metabolic pathways and environmental information processing.In addition,multiple genes related to biofilm formation were screened from the data.These findings provided important information for understanding the molecular mechanisms by which OpaR regulates biofilm formation.

关 键 词：副溶血弧菌 调控元 转录组 OpaR 

分 类 号：R378[医药卫生—病原生物学]