过敏性猝死大鼠血清IgE、MCT及咽喉和胃壁组织内P物质的检测及意义  

Detection and Significance of IgE and MCT in the Serum,and Substance P in the Throat and Gastrontestinal Tissues of Rats with Sudden Allergic Death

作　　者：李龙美 张兰兰 窦贵 LI Longmei;ZHANG Lanlan;DOU Gui(Guizhou Mingjian Judicial Appraisal Center,Guiyang 550081,China)

机构地区：[1]贵州名鉴司法鉴定中心,贵州贵阳550081

出　　处：《工业微生物》2024年第4期167-169,共3页Industrial Microbiology

摘　　要：本文旨在探讨过敏性猝死大鼠血清中的IgE、肥大细胞类胰蛋白酶(Mast cell trypsin,MCT)及咽喉、胃壁、肠壁组织中P物质(substance P,SP)的含量检测及其意义。实验选择健康清洁级SD大白鼠14只,随机分为致敏组和0.9%氯化钠溶液对照组,每组7只。采用酶联免疫吸附试验ELISA法检测大鼠血清的IgE、MCT含量,并采用免疫组化法检测咽喉和胃肠组织的P物质含量。结果显示,过敏会导致猝死大鼠血清中IgE和MCT含量升高,与对照组大鼠之间的差异具有统计学意义(P<0.05)。致敏组与对照组在咽喉黏膜、胃肠组织中P物质含量的差异具有统计学意义(P<0.05)。血清IgE、MCT和咽喉、胃肠黏膜组织的P物质含量可以作为过敏性休克大鼠法医病理学诊断的检测指标。This paper aims to explore the detection and significance of IgE,mast cell trypsin(MCT)in serum,and substance P(SP)in the throat and gastrointestinal tissues of rats with sudden allergic death.Fourteen healthy and clean SD rats were selected and divided randomly into a sensitization group and a 0.9%soduim chloride solution control group,with seven rats in each group.Enzyme linked immunosorbent assay(ELISA)was used to detect IgE and MCT levels in rat serum,while immunohistoch-emistry was used to detect the SP levels in throat and gastrointestinal tissues.The results showed that IgE and MCT levels in the serum of rats with sudden allergic death increased,with the significant difference compared with the normal group(P<0.05).The differences of SP levels in the throat and gastrointestinal tissues between the experimental group and the normal group was statistically significant(P<0.05).The IgE and MCT in the setum,and the SP in the throat and gastrointestinal tissues can be used as forensic pathological diagnostic indicators for allergic shock in rats.

关 键 词：过敏 猝死 IGE MCT 咽喉和胃壁组织 P物质 

分 类 号：D919.1[医药卫生—法医学]