姜GST基因家族成员鉴定与表达分析  

Identification and Expression Pattern Analysis of GST in Zingiber officinale

作　　者：王艳红 席克勇 田野 刘德麒 周克贵 尹军良 刘奕清 朱永兴 WANG Yanhong;XI Keyong;TIAN Ye;LIU Deqi;ZHOU Kegui;YIN Junliang;LIU Yiqing;ZHU Yongxing(College of Horticulture and Gardening,Spice Crops Research Institute,Yangtze University,Jingzhou,Hubei 434025,China;College of Agriculture,Yangtze University,Jingzhou,Hubei 434025,China)

机构地区：[1]长江大学园艺园林学院,香辛作物研究院,湖北荆州434025 [2]长江大学农学院,湖北荆州434025

出　　处：《园艺学报》2024年第8期1803-1822,共20页Acta Horticulturae Sinica

基　　金：湖北省国际科技合作计划项目(2024EHB559);湖北省重点研发计划项目(2022BBA0061)。

摘　　要：为解析姜(Zingiber officinale Roscoe)谷胱甘肽硫转移酶(glutathione-S-transferase,GST)基因家族的信息,基于姜基因组数据,分析鉴定了ZoGST家族成员及其表达模式,并进一步利用qRT-PCR检测了10个ZoGST在不同胁迫下的表达特性。在姜基因组中共鉴定到58个GST家族成员,其编码蛋白的氨基酸长度在110~675 aa之间,分子量12.21~72.51 kD,等电点4.55~9.51。系统进化树显示,ZoGST可分为Ⅰ~Ⅹ亚家族,第Ⅶ和Ⅹ亚家族成员居多数;ZoGST家族成员分布在11条染色体上,共线性分析表明该家族成员存在6对片段重复;顺式作用调控元件分析发现上游启动子区含有与光响应、逆境胁迫、激素调节相关的作用元件;转录组测序数据分析显示,ZoGST有一定的组织表达特异性且响应病害、低温等逆境胁迫,其中,ZoGSTDHAR5、ZoGSTF9、ZoGSTF13等在姜不同生长时期、不同部位、低温和病害胁迫下均有较高表达。qRT-PCR分析表明,盐胁迫下ZoGSTDHAR2(除根茎外)、ZoGSTEF1G2、ZoGSTF9在姜不同组织表达量均显著下调;淹水胁迫下,ZoGSTDHAR2等在叶和根茎中表达量显著上调;干旱胁迫下,ZoGSTF9等在根中表达量显著上调。To analyze the information of the glutathione-S-transferase(GST)gene family in ginger(Zingiber officinale Roscoe.),based on ginger genome data,the members and expression patterns of the ZoGST family were identified and analyzed.Furthermore,the expression characteristics of 10 ZoGST under different stress conditions were detected using qRT-PCR.A total of 58 members of the GST family were identified in the ginger genome,encoding proteins with amino acid lengths ranging from 110 to 675 aa,molecular weights ranging from 12.21 to 72.51 kD,isoelectric points ranging from 4.55 to 9.51.The phylogenetic tree shows that ZoGST can be divided intoⅠ–Ⅹsubfamilies,with members from theⅦandⅩsubfamilies being the majority.The members of the ZoGST family are distributed on 11 chromosomes,and collinearity analysis shows that there are 6 pairs of segment duplications in this family member.Analysis of cis-acting regulatory elements revealed that the upstream promoter region contains regulatory elements related to light response,stress,and hormone regulation.Transcriptome sequencing data analysis shows that ZoGST has certain tissue expression specificity and responds to stress such as diseases and low temperatures.Among them,ZoGSTDHAR5,ZoGSTF9,ZoGSTF13,etc.are highly expressed in ginger at different growth stages,different parts,low temperatures,and disease stresses.qRT-PCR analysis showed that under salt stress,the expression levels of ZoGSTDHAR2(except for rhizomes),ZoGSTEF1G2,and ZoGSTF9 were significantly downregulated in different tissues of ginger.Under flooding stress,the expression levels of ZoGSTDHAR2 and others were significantly upregulated in leaves and rhizome.Under drought stress,the expression levels of ZoGSTF9 and others were significantly upregulated in roots.

关 键 词：姜 GST 基因家族 表达模式 

分 类 号：S632.5[农业科学—蔬菜学]